Construct: ORF TRCN0000473506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012868.1_s317c1
- Derived from:
- ccsbBroadEn_12349
- DNA Barcode:
- ACCCAGGCTATCAGGATCTATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF150 (57484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57484 | RNF150 | ring finger protein 150 | XM_011532147.2 | 75.5% | 67.1% | (many diffs) |
| 2 | human | 57484 | RNF150 | ring finger protein 150 | XM_011532148.3 | 74.4% | 66.8% | (many diffs) |
| 3 | human | 57484 | RNF150 | ring finger protein 150 | XM_017008475.1 | 72.8% | 69.1% | (many diffs) |
| 4 | human | 57484 | RNF150 | ring finger protein 150 | NM_020724.2 | 71.5% | 70.7% | (many diffs) |
| 5 | human | 57484 | RNF150 | ring finger protein 150 | XM_005263150.5 | 68.1% | 67.3% | (many diffs) |
| 6 | human | 57484 | RNF150 | ring finger protein 150 | XM_017008476.1 | 67.8% | 66.8% | (many diffs) |
| 7 | mouse | 330812 | Rnf150 | ring finger protein 150 | XM_017312866.1 | 80.2% | 82.7% | (many diffs) |
| 8 | mouse | 330812 | Rnf150 | ring finger protein 150 | NM_177378.4 | 66% | 68.1% | (many diffs) |
| 9 | mouse | 330812 | Rnf150 | ring finger protein 150 | XM_017312865.1 | 64.1% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1011
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagctcg gcccacgacc gcctggcctg cgaccccaac accaagttcg 121 ccgccccgac ccgcggcaag aactggatag ccctcatccc caagggcaac tgcacgtaca 181 gggataagat ccggaacgcg ttcctgcaga acgcctcagc cgtggtcatc ttcaacgtgg 241 gctccaacac caacgagacc atcaccatgc cccacgcggg tgtagaagac atcgtggcca 301 taatgattcc tgagccaaaa gggaaggaga tagtaagcct gctggaaaga aacatcaccg 361 tgacaatgta catcaccatc ggaacccgga acttgcagaa atatgtgagc cgcacttcgg 421 ttgtgtttgt ctccatctcc ttcattgtcc tgatgatcat ttccctcgca tggctcgtct 481 tttattacat ccagaggttt cgatatgcaa atgccaggga taggaaccag cgccgactgg 541 gggatgcagc aaagaaAGCC ATCAGCAAAC TCCAGATCAG GACCATCAAG AAGGGTGACA 601 AGGAAACAGA GTCTGATTTT GACAACTGTG CAGTTTGTAT TGAAGGGTAC AAGCCCAATG 661 ACGTTGTCCG GATCCTGCCC TGCCGGCATC TTTTCCACAA GTCCTGTGTT GACCCCTGGC 721 TTCTAGACCA TCGTACCTGT CCCATGTGCA AGATGAACAT TCTTAAAGCC CTAGGGATCC 781 CGCCCAATGC CGACTGCATG GACGACTTGC CCACTGACTT CGAGGGCTCT CTGGGAGGTC 841 CACCCACCAA CCAGATCACA GGTGCCAGCG ACACAACAGT GAATGAAAGT TCAGTCACTT 901 TGGACCCTGC TGTCCGGACT GTGGGAGCCT TGCAGGTGGT CCAGGATACA GACCCCATCC 961 CCCAGGAGGG AGACGTCATC TTTACTACTA ACAGTGAGTT CTGCCATACT TTGCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAACCC AGGCTATCAG GATCTATATA ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt