Construct: ORF TRCN0000473525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008103.1_s317c1
- Derived from:
- ccsbBroadEn_04127
- DNA Barcode:
- TGCCTCACACACTTATAGATCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf49 (79794)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79794 | C12orf49 | chromosome 12 open reading ... | NM_024738.4 | 100% | 100% | |
| 2 | human | 79794 | C12orf49 | chromosome 12 open reading ... | XM_024449189.1 | 80.7% | 71.9% | 1_67del;112_113ins64 |
| 3 | human | 79794 | C12orf49 | chromosome 12 open reading ... | NM_001353623.2 | 67.1% | 51.4% | 1_67del;177_178ins157 |
| 4 | human | 79794 | C12orf49 | chromosome 12 open reading ... | NM_001353624.2 | 49.7% | 49.7% | 109_110ins309 |
| 5 | human | 79794 | C12orf49 | chromosome 12 open reading ... | NM_001353625.2 | 31.2% | 31.2% | 108_109ins423 |
| 6 | mouse | 76792 | 2410131K14Rik | RIKEN cDNA 2410131K14 gene | NM_001081236.1 | 85.7% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaacctggcg gccatggtgt ggcgccggct tctgcggaag aggtgggtgc 121 tcgccctggt cttcgggctg tcgctcgtct acttcctcag cagcaccttc aagcaggagg 181 agagggcagt gagagatagg aatctccTCC AGGTTCATGA CCATAATCAG CCCATCCCGT 241 GGAAAGTGCA GTTTAACTTG GGCAATAGCA GTCGTCCGAG CAATCAGTGC CGCAACTCCA 301 TTCAAGGGAA GCACCTCATC ACGGATGAAC TCGGCTACGT TTGCGAGAGG AAGGATTTGC 361 TGGTAAATGG CTGCTGTAAT GTCAACGTCC CTAGCACGAA GCAGTACTGC TGTGATGGCT 421 GCTGGCCCAA CGGCTGCTGC AGCGCCTATG AGTACTGTGT CTCCTGCTGC CTGCAGCCCA 481 ACAAGCAACT TCTCCTGGAG CGCTTCCTCA ACCGGGCAGC CGTGGCATTC CAGAACCTCT 541 TCATGGCAGT CGAAGATCAC TTTGAGTTGT GCCTGGCCAA ATGCAGGACC TCATCTCAGA 601 GCGTGCAGCA TGAGAACACC TACCGGGACC CCATAGCAAA GTATTGCTAT GGAGAAAGCC 661 CGCCCGAGCT CTTCCCCGCT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCC TCACACACTT 841 ATAGATCAGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt