Construct: ORF TRCN0000473557

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018196.1_s317c1
Derived from:
ccsbBroadEn_04313
DNA Barcode:
GCAATCGGCAAAAGCGCAATCTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NCALD (83988)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473557
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 83988 NCALD neurocalcin delta NM_001040624.1 100% 100%
2 human 83988 NCALD neurocalcin delta NM_001040625.1 100% 100%
3 human 83988 NCALD neurocalcin delta NM_001040626.1 100% 100%
4 human 83988 NCALD neurocalcin delta NM_001040627.1 100% 100%
5 human 83988 NCALD neurocalcin delta NM_001040628.1 100% 100%
6 human 83988 NCALD neurocalcin delta NM_001040629.1 100% 100%
7 human 83988 NCALD neurocalcin delta NM_001040630.1 100% 100%
8 human 83988 NCALD neurocalcin delta NM_032041.3 100% 100%
9 human 83988 NCALD neurocalcin delta XM_011517332.3 100% 100%
10 human 83988 NCALD neurocalcin delta XM_011517333.2 100% 100%
11 human 83988 NCALD neurocalcin delta XM_011517334.2 100% 100%
12 human 83988 NCALD neurocalcin delta XM_011517335.2 100% 100%
13 human 83988 NCALD neurocalcin delta XM_017013901.1 100% 100%
14 human 83988 NCALD neurocalcin delta XM_024447296.1 100% 100%
15 human 83988 NCALD neurocalcin delta XM_024447297.1 100% 100%
16 human 83988 NCALD neurocalcin delta XM_024447298.1 100% 100%
17 human 83988 NCALD neurocalcin delta XM_024447299.1 100% 100%
18 human 83988 NCALD neurocalcin delta XM_024447300.1 100% 100%
19 human 83988 NCALD neurocalcin delta XM_024447301.1 100% 100%
20 human 83988 NCALD neurocalcin delta XM_024447302.1 100% 100%
21 human 83988 NCALD neurocalcin delta XM_024447303.1 100% 100%
22 human 83988 NCALD neurocalcin delta XM_024447304.1 100% 100%
23 human 3241 HPCAL1 hippocalcin like 1 NM_001258357.1 80.5% 90.6% (many diffs)
24 human 3241 HPCAL1 hippocalcin like 1 NM_001258358.1 80.5% 90.6% (many diffs)
25 human 3241 HPCAL1 hippocalcin like 1 NM_001258359.1 80.5% 90.6% (many diffs)
26 human 3241 HPCAL1 hippocalcin like 1 NM_002149.4 80.5% 90.6% (many diffs)
27 human 3241 HPCAL1 hippocalcin like 1 NM_134421.2 80.5% 90.6% (many diffs)
28 human 3241 HPCAL1 hippocalcin like 1 XM_005246160.1 80.5% 90.6% (many diffs)
29 human 3241 HPCAL1 hippocalcin like 1 XM_005246161.1 80.5% 90.6% (many diffs)
30 human 3241 HPCAL1 hippocalcin like 1 XM_005246162.1 80.5% 90.6% (many diffs)
31 human 3241 HPCAL1 hippocalcin like 1 XM_005246163.1 80.5% 90.6% (many diffs)
32 human 3241 HPCAL1 hippocalcin like 1 XM_011510346.2 80.5% 90.6% (many diffs)
33 human 3241 HPCAL1 hippocalcin like 1 XM_011510347.1 80.5% 90.6% (many diffs)
34 human 3241 HPCAL1 hippocalcin like 1 XM_011510348.1 80.5% 90.6% (many diffs)
35 human 3241 HPCAL1 hippocalcin like 1 XM_017003950.1 80.5% 90.6% (many diffs)
36 human 3241 HPCAL1 hippocalcin like 1 XM_017003951.1 80.5% 90.6% (many diffs)
37 human 3241 HPCAL1 hippocalcin like 1 XM_017003952.1 80.5% 90.6% (many diffs)
38 human 3241 HPCAL1 hippocalcin like 1 XM_017003953.2 80.5% 90.6% (many diffs)
39 mouse 52589 Ncald neurocalcin delta NM_001170866.1 92.5% 100% (many diffs)
40 mouse 52589 Ncald neurocalcin delta NM_001170867.1 92.5% 100% (many diffs)
41 mouse 52589 Ncald neurocalcin delta NM_001170868.1 92.5% 100% (many diffs)
42 mouse 52589 Ncald neurocalcin delta NM_134094.4 92.5% 100% (many diffs)
43 mouse 52589 Ncald neurocalcin delta XM_006520107.3 92.5% 100% (many diffs)
44 mouse 52589 Ncald neurocalcin delta XM_006520108.1 92.5% 100% (many diffs)
45 mouse 52589 Ncald neurocalcin delta XM_006520109.2 92.5% 100% (many diffs)
46 mouse 52589 Ncald neurocalcin delta XM_006520111.3 92.5% 100% (many diffs)
47 mouse 52589 Ncald neurocalcin delta XM_006520112.3 92.5% 100% (many diffs)
48 mouse 52589 Ncald neurocalcin delta XM_006520113.3 92.5% 100% (many diffs)
49 mouse 52589 Ncald neurocalcin delta XM_011245362.2 92.5% 100% (many diffs)
50 mouse 52589 Ncald neurocalcin delta XM_011245363.2 92.5% 100% (many diffs)
51 mouse 52589 Ncald neurocalcin delta XM_011245364.2 92.5% 100% (many diffs)
52 mouse 52589 Ncald neurocalcin delta XM_011245365.2 92.5% 100% (many diffs)
53 mouse 52589 Ncald neurocalcin delta XM_011245366.2 92.5% 100% (many diffs)
54 mouse 52589 Ncald neurocalcin delta XM_017316691.1 92.5% 100% (many diffs)
55 mouse 52589 Ncald neurocalcin delta XM_017316692.1 92.5% 100% (many diffs)
56 mouse 52589 Ncald neurocalcin delta XM_017316693.1 92.5% 100% (many diffs)
57 mouse 52589 Ncald neurocalcin delta XM_017316694.1 92.5% 100% (many diffs)
58 mouse 52589 Ncald neurocalcin delta XM_017316695.1 92.5% 100% (many diffs)
59 mouse 52589 Ncald neurocalcin delta XM_017316696.1 92.5% 100% (many diffs)
60 mouse 52589 Ncald neurocalcin delta XM_017316697.1 92.5% 100% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
645
ORF length:
579
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg gaaacagaac agcaagctgc gcccggaggt catgcaggac ttgctggaaa
121gcacagactt tacagagcat gagatccagg aatggtataa aggcttcttg agagactgcc
181ccagtggaca tttgtcaatg gaagagttta agaaaatata tgggaacttt ttcccttatg
241gggatgcttc caaatttgca gagcatgtct tccgcaccTT CGATGCAAAT GGAGATGGGA
301CAATAGACTT TAGAGAATTC ATCATCGCCT TGAGTGTAAC TTCGAGGGGG AAGCTGGAGC
361AGAAGCTGAA ATGGGCCTTC AGCATGTACG ACCTGGACGG AAATGGCTAT ATCAGCAAGG
421CAGAGATGCT AGAGATCGTG CAGGCAATCT ATAAGATGGT TTCCTCTGTA ATGAAAATGC
481CTGAAGATGA GTCAACCCCA GAGAAAAGAA CAGAAAAGAT CTTCCGCCAG ATGGACACCA
541ATAGAGACGG AAAACTCTCC CTGGAAGAGT TCATCCGAGG AGCCAAAAGC GACCCGTCCA
601TTGTGCGCCT CCTGCAGTGC GACCCGAGCA GTGCCGGCCA GTTCTGCCCA ACTTTCTTGT
661ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
721AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
781GAAAGGACGA GCAATCGGCA AAAGCGCAAT CTTGACGCGT TAAGTCgaca atcaacctct
841ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)