Construct: ORF TRCN0000473559

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015902.1_s317c1
Derived from:
ccsbBroadEn_12236
DNA Barcode:
AACGGTCTGATACTATGCAAGGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DOCK10 (55619)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473559
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55619 DOCK10 dedicator of cytokinesis 10 NM_001290263.2 24.8% 24.8% 1_4914del
2 human 55619 DOCK10 dedicator of cytokinesis 10 NM_014689.3 24.7% 24.7% 1_4932del
3 human 55619 DOCK10 dedicator of cytokinesis 10 NM_001363762.1 24.6% 24.6% 1_4971del
4 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004446.2 23.9% 23.1% (many diffs)
5 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004447.1 23.6% 23.2% 1_4944del;6456_6531del;6591_6592ins55
6 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004445.2 23.5% 22.8% (many diffs)
7 human 55619 DOCK10 dedicator of cytokinesis 10 XM_006712617.4 23.5% 23.1% 1_4971del;6483_6558del;6618_6619ins55
8 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004444.2 23.5% 22.8% (many diffs)
9 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004443.2 23.5% 22.7% (many diffs)
10 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004442.2 23.4% 22.7% (many diffs)
11 human 55619 DOCK10 dedicator of cytokinesis 10 XM_017004441.2 23.3% 22.6% (many diffs)
12 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_011238683.2 26.2% 28.1% (many diffs)
13 mouse 210293 Dock10 dedicator of cytokinesis 10 NM_001285927.1 21.8% 23.8% (many diffs)
14 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496476.3 21.8% 23.8% (many diffs)
15 mouse 210293 Dock10 dedicator of cytokinesis 10 NM_175291.4 21.7% 23.6% (many diffs)
16 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496470.3 21.6% 23.6% (many diffs)
17 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_017319533.1 20.9% 22.6% (many diffs)
18 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_017319527.1 20.8% 22.7% (many diffs)
19 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496473.3 20.8% 22.5% (many diffs)
20 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_017319532.1 20.8% 22.5% (many diffs)
21 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496469.3 20.8% 22.7% (many diffs)
22 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496471.3 20.4% 21.9% (many diffs)
23 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496466.3 20.4% 21.9% (many diffs)
24 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496465.3 20.4% 21.8% (many diffs)
25 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496464.3 20.3% 21.8% (many diffs)
26 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496463.3 20.3% 21.8% (many diffs)
27 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_017319519.1 20.2% 21.7% (many diffs)
28 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496475.3 19.5% 21.5% (many diffs)
29 mouse 210293 Dock10 dedicator of cytokinesis 10 XR_001783679.1 19.1% (many diffs)
30 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496468.3 19.1% 20.7% (many diffs)
31 mouse 210293 Dock10 dedicator of cytokinesis 10 XM_006496467.3 19.1% 20.6% (many diffs)
32 mouse 210293 Dock10 dedicator of cytokinesis 10 XR_373539.3 12.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1692
ORF length:
1626
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa aaacagcaat ttcccagcag aggtgaagga cctgactaag cgtataagga
121ctgttttgat ggccacagct cagatgaagg agcacgagaa ggaccccgag atgctggtgg
181atctccagta cagcctggca aactcctacg caagcactcc tgaactacgc aggacctggc
241tggaaagtat ggccaagatt catgccagaa acggagattt atctgaggct gccatgtgtt
301acatccatat tgctgctctc attgcagagt atctgaaaag aaagggttac tggaaagtgg
361aaaagatttg cacagcatcc ctgctctcgg aggataccca cccctgtgat agcaactcat
421tactaacaac tcccagtgga ggaagcatgt tctctatggg atggccagct tttttgagca
481ttacaccaaa cattaaggaa gaaggagcga tgaaagagga ttctggaatg caagatacac
541catacaatga gaatatcctg gtggagcagc tatacatgtg tgtggagttt ctctggaagt
601ctgagcgata tgaactcatt gctgatgtca acaagcccat cattgctgtc tttgagaaac
661aacgagactt caaaaaattg tcagatctct actacgacat tcatcggtca tatctgaaag
721tggcagaggt ggtgaattcg gagaagcggc tgtttggtcg ctactatcgt gtggcatttt
781atgggcaggg cttttttgaa gaagaagaag gtaaagagta tatttataaa gagcctaagc
841tgacaggtct gtccgagatt tcccaaagat tactcaagct ctatgcagat aaatttggag
901cagacaatgt gaagataatc caggattcca acaaggtaaa ccccaaggat ttggacccca
961aatatgccta catccaggtg acctatgtga cgccgttctt tgaggaaaag gaaatcgaag
1021accggaagac agatttcgaa atgcaccaca acatcaaccg ctttgtcttc gagacaccct
1081tcacgctgtc gggcaagaag cacggtgggg tggcggagca gtgcaagcgg cggacgatcc
1141tgacaacgag tcacctgttc ccctacgtga agaagagaat acaagtaatt agccaatcga
1201gcacagaact gaatccaatt gaagtggcaa ttgacgagat gtccaagaag gtttctgagc
1261ttaatcagct ttGCACAATG GAAGAAGTGG ACATGATCAG ACTGCAGCTC AAACTGCAAG
1321GAAGTGTCAG CGTGAAGGTT AATGCTGGGC CAATGGCCTA TGCACGAGCT TTTCTTGAAG
1381AAACCAATGC AAAGAAGTAC CCTGACAACC AAGTAAAGCT TTTGAAGGAG ATCTTCAGGC
1441AATTTGCAGA TGCATGTGGG CAGGCCCTTG ACGTGAATGA GCGCCTCATC AAAGAGGACC
1501AGCTGGAGTA CCAGGAAGAA CTGAGGTCCC ACTACAAGGA CATGCTCAGC GAACTCTCCA
1561CAGTCATGAA TGAGCAGATT ACGGGCAGGG ACGACCTGTC AAAGCGCGGA GTGGACCAAA
1621CCTGCACTCG AGTAATTAGC AAAGCAACTC CGGCCCTACC CACGGTCTCC ATCTCATCTA
1681GTGCTGAAGT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1741ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1801CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC GGTCTGATAC TATGCAAGGT
1861CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)