Construct: ORF TRCN0000473590
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004967.1_s317c1
- Derived from:
- ccsbBroadEn_15898
- DNA Barcode:
- ATTAAAAGAGAGTTTAGGGCGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETNK1 (55500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473590
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55500 | ETNK1 | ethanolamine kinase 1 | NM_001039481.2 | 100% | 100% | |
| 2 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_005253420.4 | 55.4% | 52.7% | (many diffs) |
| 3 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_017019581.1 | 47% | 43.9% | (many diffs) |
| 4 | human | 55500 | ETNK1 | ethanolamine kinase 1 | NM_018638.5 | 43.7% | 40.7% | (many diffs) |
| 5 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XM_017019580.1 | 37.4% | 34.8% | (many diffs) |
| 6 | human | 55500 | ETNK1 | ethanolamine kinase 1 | XR_002957349.1 | 6.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caattacatc cacgtccctc ccggctcccc ggaggtgccc aagctgaacg 121 tcaccgttca ggatcaggag gagcatcgct gccgggaggg ggccctgagc ctcctgcaac 181 acctgcggcc TCACTGGGAC CCCCAGGAGG TGACCCTGCA GCTCTTCACA GATGGAATCA 241 CAAATAAACT TATTGGCTGT TACGTGGGAA ACACCATGGA GGATGTAGTC CTGGTGAGAA 301 TTTATGGCAA TAAGACTGAG TTATTAGTCG ATCGAGATGA GGAAGTAAAG AGTTTTCGAG 361 TGTTGCAGGC TCATGGGTGT GCACCACAAC TCTACTGTAC CTTCAATAAT GGACTATGCT 421 ATGAATTTAT ACAAGGAGAA GCACTGGATC CAAAGCATGT CTGCAACCCA GCCATTTTCA 481 GTTTATCATC GTTGACTCTT TGCAAAGGAA AAACTACAAG ATGTTTTGGA TTAACCGGCT 541 GCAGAGGGTC AAGGCTTCTG CTTAGTTTTT TCTACCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 721 TAAAAGAGAG TTTAGGGCGG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt