Transcript: Human NM_018638.5

Homo sapiens ethanolamine kinase 1 (ETNK1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
ETNK1 (55500)
Length:
7063
CDS:
261..1352

Additional Resources:

NCBI RefSeq record:
NM_018638.5
NBCI Gene record:
ETNK1 (55500)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148309 GTTATTAGTCGATCGAGATG pXPR_003 AGG 271 25% 2 0.396 ETNK1 ETNK1 75531
2 BRDN0001148358 CATTGTGTGCATGAATAGCA pXPR_003 TGG 449 41% 3 -0.0747 ETNK1 ETNK1 75532
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_018638.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010246 CTGAGTTATTAGTCGATCGAG pLKO.1 511 CDS 100% 2.640 3.696 N ETNK1 n/a
2 TRCN0000273300 CTGAGTTATTAGTCGATCGAG pLKO_005 511 CDS 100% 2.640 3.696 N ETNK1 n/a
3 TRCN0000196647 GATTTGCAGATGAAGACATTA pLKO.1 790 CDS 100% 13.200 9.240 N ETNK1 n/a
4 TRCN0000196531 GCATTGATCTTGTACACTTAA pLKO.1 2480 3UTR 100% 13.200 9.240 N ETNK1 n/a
5 TRCN0000273243 TATGCCTCTAAACAATCAAAT pLKO_005 1475 3UTR 100% 13.200 9.240 N ETNK1 n/a
6 TRCN0000273301 CTTACCTTGAAGCCTACAAAG pLKO_005 1108 CDS 100% 10.800 7.560 N ETNK1 n/a
7 TRCN0000284943 CTTTGCCATAATGACCTATTG pLKO_005 909 CDS 100% 10.800 7.560 N ETNK1 n/a
8 TRCN0000010245 CCTGGTGAGAATTTATGGCAA pLKO.1 485 CDS 100% 2.640 1.848 N ETNK1 n/a
9 TRCN0000010243 CCAATTACATCCACGTCCCTC pLKO.1 265 CDS 100% 2.160 1.512 N ETNK1 n/a
10 TRCN0000010244 AGGTGCCCAAGCTGAACGTCA pLKO.1 298 CDS 100% 0.880 0.616 N ETNK1 n/a
11 TRCN0000024557 CCTGGCATATGATATTGGAAA pLKO.1 1004 CDS 100% 0.000 0.000 N Etnk1 n/a
12 TRCN0000010247 GAATTTATACAAGGAGAAGCA pLKO.1 618 CDS 100% 2.640 1.584 N ETNK1 n/a
13 TRCN0000273244 GAATTTATACAAGGAGAAGCA pLKO_005 618 CDS 100% 2.640 1.584 N ETNK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_018638.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03591 pDONR223 100% 80.3% 80.3% None 0_1ins267 n/a
2 ccsbBroad304_03591 pLX_304 0% 80.3% 80.3% V5 0_1ins267 n/a
3 TRCN0000467028 CGACTAAGAACAGAGCAATGAGGG pLX_317 31.6% 80.3% 80.3% V5 0_1ins267 n/a
4 ccsbBroadEn_15099 pDONR223 0% 80.3% 80.3% None 0_1ins267 n/a
5 ccsbBroad304_15099 pLX_304 0% 80.3% 80.3% V5 0_1ins267 n/a
6 TRCN0000470683 TTCTCTCAGCTAGTTGTATTGAAT pLX_317 33.3% 80.3% 80.3% V5 0_1ins267 n/a
7 ccsbBroadEn_15898 pDONR223 0% 43.7% 40.7% None (many diffs) n/a
8 ccsbBroad304_15898 pLX_304 0% 43.7% 40.7% V5 (many diffs) n/a
9 TRCN0000473590 ATTAAAAGAGAGTTTAGGGCGGCA pLX_317 74.5% 43.7% 40.7% V5 (many diffs) n/a
10 TRCN0000489670 ATAAGCTCGGCGCCGATCATACCC pLX_317 75% 43.7% 40.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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