Transcript: Human XM_005253420.4

PREDICTED: Homo sapiens ethanolamine kinase 1 (ETNK1), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ETNK1 (55500)
Length:
1158
CDS:
20..841

Additional Resources:

NCBI RefSeq record:
XM_005253420.4
NBCI Gene record:
ETNK1 (55500)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148309 GTTATTAGTCGATCGAGATG pXPR_003 AGG 271 49% 2 0.4336 ETNK1 ETNK1 75531
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005253420.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010246 CTGAGTTATTAGTCGATCGAG pLKO.1 537 CDS 100% 2.640 3.696 N ETNK1 n/a
2 TRCN0000273300 CTGAGTTATTAGTCGATCGAG pLKO_005 537 CDS 100% 2.640 3.696 N ETNK1 n/a
3 TRCN0000010245 CCTGGTGAGAATTTATGGCAA pLKO.1 511 CDS 100% 2.640 1.848 N ETNK1 n/a
4 TRCN0000010243 CCAATTACATCCACGTCCCTC pLKO.1 291 CDS 100% 2.160 1.512 N ETNK1 n/a
5 TRCN0000010244 AGGTGCCCAAGCTGAACGTCA pLKO.1 324 CDS 100% 0.880 0.616 N ETNK1 n/a
6 TRCN0000010247 GAATTTATACAAGGAGAAGCA pLKO.1 644 CDS 100% 2.640 1.584 N ETNK1 n/a
7 TRCN0000273244 GAATTTATACAAGGAGAAGCA pLKO_005 644 CDS 100% 2.640 1.584 N ETNK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005253420.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03591 pDONR223 100% 56.6% 53.3% None (many diffs) n/a
2 ccsbBroad304_03591 pLX_304 0% 56.6% 53.3% V5 (many diffs) n/a
3 TRCN0000467028 CGACTAAGAACAGAGCAATGAGGG pLX_317 31.6% 56.6% 53.3% V5 (many diffs) n/a
4 ccsbBroadEn_15099 pDONR223 0% 56.6% 53.3% None (many diffs) n/a
5 ccsbBroad304_15099 pLX_304 0% 56.6% 53.3% V5 (many diffs) n/a
6 TRCN0000470683 TTCTCTCAGCTAGTTGTATTGAAT pLX_317 33.3% 56.6% 53.3% V5 (many diffs) n/a
7 ccsbBroadEn_15898 pDONR223 0% 55.4% 52.7% None (many diffs) n/a
8 ccsbBroad304_15898 pLX_304 0% 55.4% 52.7% V5 (many diffs) n/a
9 TRCN0000473590 ATTAAAAGAGAGTTTAGGGCGGCA pLX_317 74.5% 55.4% 52.7% V5 (many diffs) n/a
10 TRCN0000489670 ATAAGCTCGGCGCCGATCATACCC pLX_317 75% 55.4% 52.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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