Transcript: Human XR_002957349.1

PREDICTED: Homo sapiens ethanolamine kinase 1 (ETNK1), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ETNK1 (55500)
Length:
7192
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002957349.1
NBCI Gene record:
ETNK1 (55500)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002957349.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010246 CTGAGTTATTAGTCGATCGAG pLKO.1 537 3UTR 100% 2.640 3.696 N ETNK1 n/a
2 TRCN0000273300 CTGAGTTATTAGTCGATCGAG pLKO_005 537 3UTR 100% 2.640 3.696 N ETNK1 n/a
3 TRCN0000196647 GATTTGCAGATGAAGACATTA pLKO.1 816 3UTR 100% 13.200 9.240 N ETNK1 n/a
4 TRCN0000196531 GCATTGATCTTGTACACTTAA pLKO.1 2609 3UTR 100% 13.200 9.240 N ETNK1 n/a
5 TRCN0000273243 TATGCCTCTAAACAATCAAAT pLKO_005 1604 3UTR 100% 13.200 9.240 N ETNK1 n/a
6 TRCN0000273301 CTTACCTTGAAGCCTACAAAG pLKO_005 1237 3UTR 100% 10.800 7.560 N ETNK1 n/a
7 TRCN0000284943 CTTTGCCATAATGACCTATTG pLKO_005 935 3UTR 100% 10.800 7.560 N ETNK1 n/a
8 TRCN0000010245 CCTGGTGAGAATTTATGGCAA pLKO.1 511 3UTR 100% 2.640 1.848 N ETNK1 n/a
9 TRCN0000010243 CCAATTACATCCACGTCCCTC pLKO.1 291 3UTR 100% 2.160 1.512 N ETNK1 n/a
10 TRCN0000010244 AGGTGCCCAAGCTGAACGTCA pLKO.1 324 3UTR 100% 0.880 0.616 N ETNK1 n/a
11 TRCN0000024557 CCTGGCATATGATATTGGAAA pLKO.1 1133 3UTR 100% 0.000 0.000 N Etnk1 n/a
12 TRCN0000010247 GAATTTATACAAGGAGAAGCA pLKO.1 644 3UTR 100% 2.640 1.584 N ETNK1 n/a
13 TRCN0000273244 GAATTTATACAAGGAGAAGCA pLKO_005 644 3UTR 100% 2.640 1.584 N ETNK1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002957349.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03591 pDONR223 100% 18.8% None 1_19del;986_1088del;1479_7192del n/a
2 ccsbBroad304_03591 pLX_304 0% 18.8% V5 1_19del;986_1088del;1479_7192del n/a
3 TRCN0000467028 CGACTAAGAACAGAGCAATGAGGG pLX_317 31.6% 18.8% V5 1_19del;986_1088del;1479_7192del n/a
4 ccsbBroadEn_15099 pDONR223 0% 18.8% None 1_19del;986_1088del;1479_7192del n/a
5 ccsbBroad304_15099 pLX_304 0% 18.8% V5 1_19del;986_1088del;1479_7192del n/a
6 TRCN0000470683 TTCTCTCAGCTAGTTGTATTGAAT pLX_317 33.3% 18.8% V5 1_19del;986_1088del;1479_7192del n/a
7 ccsbBroadEn_15898 pDONR223 0% 6.6% None (many diffs) n/a
8 ccsbBroad304_15898 pLX_304 0% 6.6% V5 (many diffs) n/a
9 TRCN0000473590 ATTAAAAGAGAGTTTAGGGCGGCA pLX_317 74.5% 6.6% V5 (many diffs) n/a
10 TRCN0000489670 ATAAGCTCGGCGCCGATCATACCC pLX_317 75% 6.6% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV