Construct: ORF TRCN0000473627
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004996.1_s317c1
- Derived from:
- ccsbBroadEn_16160
- DNA Barcode:
- ATCAATCAGAAAGTCAGCTAAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKD1P1 (339044)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473627
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | NM_001351200.1 | 100% | 100% | |
| 2 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_024452522.1 | 99.8% | 99.5% | 277C>T |
| 3 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_005255485.4 | 99.5% | 99.1% | 277C>T;364T>C;510C>T |
| 4 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_005255492.4 | 99.5% | 99.1% | 277C>T;364T>C;510C>T |
| 5 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_024450138.1 | 99.2% | 98.2% | (many diffs) |
| 6 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_024450137.1 | 90.6% | 87.2% | (many diffs) |
| 7 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_017030213.2 | 85.6% | 81.1% | (many diffs) |
| 8 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_024452521.1 | 81% | 73.6% | (many diffs) |
| 9 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | XM_011522336.3 | 80% | 77.7% | (many diffs) |
| 10 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_006720917.4 | 79.7% | 76.4% | (many diffs) |
| 11 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522581.1 | 79.7% | 76.4% | (many diffs) |
| 12 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522583.3 | 79.7% | 76.4% | (many diffs) |
| 13 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_011522593.1 | 79.7% | 76.4% | (many diffs) |
| 14 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_011522594.3 | 79.7% | 76.4% | (many diffs) |
| 15 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_024450139.1 | 76.3% | 72.9% | (many diffs) |
| 16 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522584.2 | 75.4% | 69.3% | (many diffs) |
| 17 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_011522595.2 | 75.4% | 69.3% | (many diffs) |
| 18 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XR_001756353.1 | 68.7% | (many diffs) | |
| 19 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | NM_001277325.2 | 64.4% | 61.2% | (many diffs) |
| 20 | human | 9284 | NPIPA1 | nuclear pore complex intera... | NM_006985.4 | 64.3% | 61% | (many diffs) |
| 21 | human | 642778 | NPIPA3 | nuclear pore complex intera... | NM_001277323.1 | 64.2% | 60.4% | (many diffs) |
| 22 | human | 642799 | NPIPA2 | nuclear pore complex intera... | NM_001277324.1 | 64.2% | 60.4% | (many diffs) |
| 23 | human | 729978 | NPIPB2 | nuclear pore complex intera... | XM_024450430.1 | 63.4% | 53.1% | (many diffs) |
| 24 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | XM_011522329.3 | 61.7% | 58.7% | (many diffs) |
| 25 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_011522587.1 | 61.5% | 57.5% | (many diffs) |
| 26 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_024450380.1 | 61.5% | 57.5% | (many diffs) |
| 27 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_024452519.1 | 61% | 57.9% | (many diffs) |
| 28 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450387.1 | 61% | 55% | (many diffs) |
| 29 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | NM_001282507.1 | 60.7% | 57.1% | (many diffs) |
| 30 | human | 101059953 | NPIPA8 | nuclear pore complex intera... | NM_001282511.2 | 60.7% | 57.1% | (many diffs) |
| 31 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_017022834.1 | 60.7% | 57.1% | (many diffs) |
| 32 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_017022835.1 | 60.7% | 57.1% | (many diffs) |
| 33 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | XM_024450132.1 | 59% | 51.9% | (many diffs) |
| 34 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_017023554.1 | 58.8% | 51.1% | (many diffs) |
| 35 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_024450379.1 | 58.8% | 51.1% | (many diffs) |
| 36 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450385.1 | 58.8% | 51.1% | (many diffs) |
| 37 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450386.1 | 58.8% | 51.1% | (many diffs) |
| 38 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522580.2 | 58.4% | 55.2% | (many diffs) |
| 39 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450388.1 | 58.4% | 55.2% | (many diffs) |
| 40 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522578.2 | 57.3% | 51.1% | (many diffs) |
| 41 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450384.1 | 57.3% | 51.1% | (many diffs) |
| 42 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_017022833.2 | 56.6% | 51.4% | (many diffs) |
| 43 | human | 729978 | NPIPB2 | nuclear pore complex intera... | XM_024450427.1 | 56.1% | 44.7% | (many diffs) |
| 44 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | XM_011522337.1 | 55.8% | 54.2% | (many diffs) |
| 45 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_011522339.3 | 55.6% | 52.5% | (many diffs) |
| 46 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_024450136.1 | 55.6% | 53.9% | (many diffs) |
| 47 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522585.1 | 55.3% | 53.6% | (many diffs) |
| 48 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_011522596.1 | 55.3% | 53.6% | (many diffs) |
| 49 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_017030211.2 | 54.7% | 49.2% | (many diffs) |
| 50 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522576.2 | 54.6% | 49.2% | (many diffs) |
| 51 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_011522577.2 | 54.6% | 49.2% | (many diffs) |
| 52 | human | 642778 | NPIPA3 | nuclear pore complex intera... | XM_017023553.1 | 54.6% | 49.2% | (many diffs) |
| 53 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450381.1 | 54.6% | 49.2% | (many diffs) |
| 54 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450382.1 | 54.6% | 49.2% | (many diffs) |
| 55 | human | 642799 | NPIPA2 | nuclear pore complex intera... | XM_024450383.1 | 54.6% | 49.2% | (many diffs) |
| 56 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_005255735.5 | 54.5% | 48.7% | (many diffs) |
| 57 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_024452520.1 | 54.3% | 49.2% | (many diffs) |
| 58 | human | 728888 | NPIPB11 | nuclear pore complex intera... | XM_024450422.1 | 53.8% | 41.4% | (many diffs) |
| 59 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | XR_002957772.1 | 51.8% | (many diffs) | |
| 60 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XR_001756352.1 | 46.4% | (many diffs) | |
| 61 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_024452523.1 | 41.5% | 44% | (many diffs) |
| 62 | human | 728741 | NPIPB6 | nuclear pore complex intera... | XM_011545931.2 | 36.4% | 27.1% | (many diffs) |
| 63 | human | 728741 | NPIPB6 | nuclear pore complex intera... | XM_005255741.4 | 36.2% | 27.3% | (many diffs) |
| 64 | human | 728741 | NPIPB6 | nuclear pore complex intera... | XM_017023630.1 | 36.2% | 27.3% | (many diffs) |
| 65 | human | 728741 | NPIPB6 | nuclear pore complex intera... | XM_024450419.1 | 36.2% | 27.3% | (many diffs) |
| 66 | human | 728734 | NPIPB8 | nuclear pore complex intera... | XM_024450417.1 | 35.9% | 26.7% | (many diffs) |
| 67 | human | 100507607 | NPIPB9 | nuclear pore complex intera... | XM_017022825.2 | 35.6% | 26.9% | (many diffs) |
| 68 | human | 728734 | NPIPB8 | nuclear pore complex intera... | XM_017023626.1 | 35.6% | 26.9% | (many diffs) |
| 69 | human | 728734 | NPIPB8 | nuclear pore complex intera... | XM_024450416.1 | 35.6% | 26.9% | (many diffs) |
| 70 | human | 100288332 | NPIPA5 | nuclear pore complex intera... | XR_932754.3 | 34.7% | (many diffs) | |
| 71 | human | 440348 | NPIPB15 | nuclear pore complex intera... | NM_001306094.1 | 33.6% | 25.5% | (many diffs) |
| 72 | human | 440348 | NPIPB15 | nuclear pore complex intera... | XM_011523498.3 | 33.6% | 25.5% | (many diffs) |
| 73 | human | 440348 | NPIPB15 | nuclear pore complex intera... | XM_017023248.1 | 33.6% | 25.5% | (many diffs) |
| 74 | human | 100507607 | NPIPB9 | nuclear pore complex intera... | XM_017022823.2 | 32% | 24.4% | (many diffs) |
| 75 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XR_001756354.2 | 28.4% | (many diffs) | |
| 76 | human | 102724993 | LOC102724993 | nuclear pore complex-intera... | XM_017030214.2 | 28% | 25.2% | (many diffs) |
| 77 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_017022836.2 | 27.8% | 24.6% | (many diffs) |
| 78 | human | 101059938 | NPIPA7 | nuclear pore complex intera... | XM_017022840.2 | 27.8% | 24.6% | (many diffs) |
| 79 | human | 105369154 | PKD1P6-NPIPP1 | PKD1P6-NPIPP1 readthrough | NR_123722.1 | 23.1% | (many diffs) | |
| 80 | human | 728741 | NPIPB6 | nuclear pore complex intera... | XM_005255743.3 | 17.9% | 13.6% | (many diffs) |
| 81 | human | 440353 | NPIPB12 | nuclear pore complex intera... | NM_001355401.1 | 17.7% | 12.6% | (many diffs) |
| 82 | human | 440345 | NPIPB4 | nuclear pore complex intera... | XM_024450289.1 | 17.6% | 12.7% | (many diffs) |
| 83 | human | 728888 | NPIPB11 | nuclear pore complex intera... | NM_001310137.2 | 14.7% | 10.7% | (many diffs) |
| 84 | human | 100132247 | NPIPB5 | nuclear pore complex intera... | NM_001135865.1 | 14.6% | 10.4% | (many diffs) |
| 85 | human | 23117 | NPIPB3 | nuclear pore complex intera... | NM_130464.3 | 14.6% | 10.4% | (many diffs) |
| 86 | human | 613037 | NPIPB13 | nuclear pore complex intera... | NM_001321892.1 | 14.5% | 10.4% | (many diffs) |
| 87 | human | 440345 | NPIPB4 | nuclear pore complex intera... | XM_017023247.1 | 14.5% | 10.4% | (many diffs) |
| 88 | human | 440345 | NPIPB4 | nuclear pore complex intera... | XM_024450285.1 | 14.5% | 10.4% | (many diffs) |
| 89 | human | 728888 | NPIPB11 | nuclear pore complex intera... | XM_024450420.1 | 14.3% | 10.9% | (many diffs) |
| 90 | human | 440345 | NPIPB4 | nuclear pore complex intera... | XM_024450284.1 | 13.6% | 9.7% | (many diffs) |
| 91 | human | 440345 | NPIPB4 | nuclear pore complex intera... | NM_001310148.1 | 13.5% | 9.8% | (many diffs) |
| 92 | human | 440345 | NPIPB4 | nuclear pore complex intera... | XM_017023244.2 | 13.5% | 9.8% | (many diffs) |
| 93 | human | 440345 | NPIPB4 | nuclear pore complex intera... | XM_024450283.1 | 13.2% | 10% | (many diffs) |
| 94 | human | 105369154 | PKD1P6-NPIPP1 | PKD1P6-NPIPP1 readthrough | NR_123721.1 | 12.8% | (many diffs) | |
| 95 | human | 110006323 | PKD1P4-NPIPA8 | PKD1P4-NPIPA8 readthrough | NR_146336.1 | 9.2% | (many diffs) | |
| 96 | human | 109951030 | PKD1P3-NPIPA1 | PKD1P3-NPIPA1 readthrough | NR_146231.1 | 9.1% | (many diffs) | |
| 97 | human | 339044 | PKD1P1 | polycystin 1, transient rec... | NR_036447.1 | 8.8% | (many diffs) | |
| 98 | human | 110006322 | PKD1P5-LOC105376752 | PKD1P5-LOC105376752 readthr... | NR_146331.1 | 5.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 765
- ORF length:
- 696
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttttgttgc ttaggatatg aatggctgag cggaggctgt aaaacctggc 121 actctgcttg ggttatcaat actctggctg accatcgtca tcgtgggact gactttggtg 181 gaagtccttg gttacttatc attactgtgt ttctgagaag ttataaattt gccatctccc 241 tctgcacaag ttacctttgt gtgtctttcc tgaagactat cttcccgtct caaaatggac 301 atgatggatc cacggatgta cagcagagag ccaggaggtc caactgccGT AGACAGGAAG 361 GAATTAAAAT TGTCCTGGAA GACATCTTTA CTTTATGGAG ACAGGTGGAA ACCAAAGTTC 421 GAGCTAAAAT CCGTAAGATG AAGGTGACAA CAAAAGTCAA CCGTCATGAC AAAATCAATG 481 GAAAGAGGAA GACCGCCAAA GAACATCTGA GGAAACTAAG CATGAAAGAA CGTGAGCACG 541 GAGAAAAGGA GAGGCAGGTG TCAGAGGCAG AGGAAAATGG GAAATTGGAT ATGAAAGAAA 601 TACACACCTA CATGGAAATG TTTCAACGTG CGCAAGCGTT GCGGCGGCGG GCAGAGGACT 661 ACTACAGATG CAAAATCACC CCTTCTGCAA GAAAGCCTCT TTGCAACCGG TGCACTTACA 721 ATTTGGTATT ACCTGGGAGT GAAAAGAAAT ATTACAGCCA TGCCTTGCCC AACTTTCTTG 781 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 841 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 901 GGAAAGGACG AATCAATCAG AAAGTCAGCT AAGTCACGCG TTAAGTCgac aatcaacctc 961 tggattacaa aatttgtgaa agatt