Construct: ORF TRCN0000473651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004828.1_s317c1
- Derived from:
- ccsbBroadEn_00776
- DNA Barcode:
- AGCCCAGTGTGTCACCACACTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOXB6 (3216)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3216 | HOXB6 | homeobox B6 | NM_001369397.2 | 100% | 100% | |
| 2 | human | 3216 | HOXB6 | homeobox B6 | NM_018952.5 | 100% | 100% | |
| 3 | human | 3216 | HOXB6 | homeobox B6 | XM_011524727.3 | 77.7% | 77.7% | 1_192del |
| 4 | mouse | 15414 | Hoxb6 | homeobox B6 | NM_008269.1 | 91.2% | 97.7% | (many diffs) |
| 5 | mouse | 15414 | Hoxb6 | homeobox B6 | XM_006532292.3 | 66.1% | 70.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttcctatttc gtgaactcca ccttccccgt cactctggcc agcgggcagg 121 agtccttcct gggccagcta ccgctctatt cgtcgggcta tgcggacccg ctgagacatt 181 accccgcgcc ctacgggcca gggccgggcc aggacaaggg ctttgccact tcctcctatt 241 acccgccggc gggcggtggc tacggccgag cggcgccctg cgactacggg ccggcgccgg 301 ccttctaccg cgagaaagag tcggcctgcg cactctccgg cgccgacgag cagcccccgt 361 tccaccCCGA GCCGCGGAAG TCGGACTGCG CGCAGGACAA GAGCGTGTTC GGCGAGACAG 421 AAGAGCAGAA GTGCTCCACT CCGGTCTACC CGTGGATGCA GCGGATGAAT TCGTGCAACA 481 GTTCCTCCTT TGGGCCCAGC GGCCGGCGAG GCCGCCAGAC ATACACACGT TACCAGACGC 541 TGGAGCTGGA GAAGGAGTTT CACTACAATC GCTACCTGAC GCGGCGGCGG CGCATCGAGA 601 TCGCGCACGC CCTGTGCCTG ACGGAGAGGC AGATCAAGAT ATGGTTCCAG AACCGACGCA 661 TGAAGTGGAA AAAGGAGAGC AAACTGCTCA GCGCGTCTCA GCTCAGTGCC GAGGAGGAGG 721 AAGAAAAACA GGCCGAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 781 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 841 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGCCCAG TGTGTCACCA 901 CACTTCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt