Construct: ORF TRCN0000473689

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017220.1_s317c1
Derived from:
ccsbBroadEn_14285
DNA Barcode:
CCATCAGATCATGACCTAGCGTTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
TSGA10 (80705)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473689
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80705 TSGA10 testis specific 10 NM_001349012.1 99.9% 28.3% 587delA;817C>A
2 human 80705 TSGA10 testis specific 10 NM_025244.4 99.9% 28.3% 587delA;817C>A
3 human 80705 TSGA10 testis specific 10 NM_182911.4 99.9% 28.3% 587delA;817C>A
4 human 80705 TSGA10 testis specific 10 XM_005264023.1 99.9% 28.3% 587delA;817C>A
5 human 80705 TSGA10 testis specific 10 XM_005264024.2 99.9% 28.3% 587delA;817C>A
6 human 80705 TSGA10 testis specific 10 XM_005264025.2 99.9% 28.3% 587delA;817C>A
7 human 80705 TSGA10 testis specific 10 XM_005264026.1 99.9% 28.3% 587delA;817C>A
8 human 80705 TSGA10 testis specific 10 XM_005264027.1 99.9% 28.3% 587delA;817C>A
9 human 80705 TSGA10 testis specific 10 XM_005264029.1 99.9% 28.3% 587delA;817C>A
10 human 80705 TSGA10 testis specific 10 XM_006712781.2 99.9% 28.3% 587delA;817C>A
11 human 80705 TSGA10 testis specific 10 XM_011511937.1 99.9% 28.3% 587delA;817C>A
12 human 80705 TSGA10 testis specific 10 XM_011511939.1 99.9% 28.3% 587delA;817C>A
13 human 80705 TSGA10 testis specific 10 XM_011511941.1 99.9% 28.3% 587delA;817C>A
14 human 80705 TSGA10 testis specific 10 XM_011511942.1 99.9% 28.3% 587delA;817C>A
15 human 80705 TSGA10 testis specific 10 XM_017005039.1 99.9% 28.3% 587delA;817C>A
16 human 80705 TSGA10 testis specific 10 XM_017005040.2 99.9% 28.3% 587delA;817C>A
17 human 80705 TSGA10 testis specific 10 XM_017005041.2 99.9% 28.3% 587delA;817C>A
18 human 80705 TSGA10 testis specific 10 XM_017005042.1 99.9% 28.3% 587delA;817C>A
19 human 80705 TSGA10 testis specific 10 XM_017005043.2 99.9% 28.3% 587delA;817C>A
20 human 80705 TSGA10 testis specific 10 XM_017005044.1 99.9% 28.3% 587delA;817C>A
21 human 80705 TSGA10 testis specific 10 XM_017005045.1 99.9% 28.3% 587delA;817C>A
22 human 80705 TSGA10 testis specific 10 XM_017005046.1 99.9% 28.3% 587delA;817C>A
23 human 80705 TSGA10 testis specific 10 XM_017005047.1 99.9% 28.3% 587delA;817C>A
24 human 80705 TSGA10 testis specific 10 XM_017005048.1 99.9% 28.3% 587delA;817C>A
25 human 80705 TSGA10 testis specific 10 XM_024453158.1 99.9% 28.3% 587delA;817C>A
26 human 80705 TSGA10 testis specific 10 XM_024453159.1 99.9% 28.3% 587delA;817C>A
27 human 80705 TSGA10 testis specific 10 XM_024453160.1 99.9% 28.3% 587delA;817C>A
28 human 80705 TSGA10 testis specific 10 XM_024453161.1 99.9% 28.3% 587delA;817C>A
29 human 80705 TSGA10 testis specific 10 XM_011511935.3 84.4% 23.9% 1_384del;971delA;1201C>A
30 human 80705 TSGA10 testis specific 10 NM_001349013.2 84.3% 33.6% 587delA;609_610ins327
31 human 80705 TSGA10 testis specific 10 NM_001349014.1 84.3% 33.6% 587delA;609_610ins327
32 human 80705 TSGA10 testis specific 10 XM_017005035.1 78% 22.1% 1_585del;1172delA;1402C>A
33 human 80705 TSGA10 testis specific 10 XM_011511934.1 77.6% 22% 1_600del;1187delA;1417C>A
34 human 80705 TSGA10 testis specific 10 XM_017005036.2 63.1% 25.3% (many diffs)
35 human 80705 TSGA10 testis specific 10 XM_017005037.1 59.8% 26.6% (many diffs)
36 human 80705 TSGA10 testis specific 10 XM_017005038.1 59.7% 26.7% (many diffs)
37 human 80705 TSGA10 testis specific 10 NR_146001.2 52.2% (many diffs)
38 mouse 211484 Tsga10 testis specific 10 NM_207228.3 88.8% 26.9% (many diffs)
39 mouse 211484 Tsga10 testis specific 10 NM_001290721.1 87.8% 27.2% (many diffs)
40 mouse 211484 Tsga10 testis specific 10 XM_017319574.1 87.3% 26.7% (many diffs)
41 mouse 211484 Tsga10 testis specific 10 XM_017319575.1 87.3% 26.7% (many diffs)
42 mouse 211484 Tsga10 testis specific 10 XM_017319576.1 87.3% 26.7% (many diffs)
43 mouse 211484 Tsga10 testis specific 10 NM_001290720.1 79% 30.3% (many diffs)
44 mouse 211484 Tsga10 testis specific 10 XM_017319578.1 77.2% 16.3% (many diffs)
45 mouse 211484 Tsga10 testis specific 10 XM_006495822.2 69% 20.9% (many diffs)
46 mouse 211484 Tsga10 testis specific 10 XM_011238461.1 68% 20.8% (many diffs)
47 mouse 211484 Tsga10 testis specific 10 XM_006495823.3 61.4% 22.9% (many diffs)
48 mouse 211484 Tsga10 testis specific 10 XM_017319571.1 61.3% 22.5% (many diffs)
49 mouse 211484 Tsga10 testis specific 10 XM_011238463.2 53.1% 25.3% (many diffs)
50 mouse 211484 Tsga10 testis specific 10 NR_110976.1 50.3% (many diffs)
51 mouse 211484 Tsga10 testis specific 10 XM_017319577.1 47.2% 28.1% (many diffs)
52 mouse 211484 Tsga10 testis specific 10 XR_001783692.1 47.1% (many diffs)
53 mouse 211484 Tsga10 testis specific 10 XR_865225.1 44.8% (many diffs)
54 mouse 211484 Tsga10 testis specific 10 XM_017319580.1 32.2% 36.2% (many diffs)
55 mouse 211484 Tsga10 testis specific 10 XM_017319581.1 30.3% 37.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
672
ORF length:
606
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat gcgaagtagg tctaaaagtc caagacgccc atcaccaact gcccggggtg
121caaactgtga tgtagaactt ttgaagacaa caacaagaga tcgtgaagaa cttaaatgca
181tgctggaaaa atatgagcgc catttggcag aaattcaggg taatgtcaag gttcttaaat
241ctgagagaga caagatcttc cttctttatg aacaggcaca ggaagaaatt acccgacttc
301gacgagaaat gatgaaaagc tgtaagagtc ctaaatcaac aacggcacat gctattctcc
361ggcgagtgga gactgaaaga gatgtagcct ttactgattt acgaagaatg accacagaac
421gagatagtct aagggagagg ctaaagattg ctcaagagac agcatttaat gagaaggctc
481acctggaaca aaggatagag gagctggagt gtacagttca taatcttgat gatgaacgta
541tggagcaaat gtcaaatatg actttgatga aggaaaccat aagcactgtg gaaaaagaaa
601tgaaatcact agcaagaaag gcaatggata ccgaaagtga acttggcaga caaaagcaga
661gaataattct ttgagacttt tgtatgaaaa cacagaaaaa gatctttctg atactcagcg
721acaccttgct aagaaaaaat atgagctaca gcttactcag gagaaaatta tgtgcttgga
781tgaaaaaatt gataacttta caaggcaaaa tattgcacag cgagaagaaa tcagcattct
841tggtggaacc ctcaatgatc tggctaaaga aaaggaatgc atgcaagcat gtttggataa
901aaaatctgag aatattgcat cccttggaga gagtttggca atgaaagaaa agaccatttc
961aggcatgaag aatatcattg ctgagatgga acaggcatca agacagtgta ctgaggccct
1021aattgtgtgt gaacaagacg tttccagaat gcgtcggcaa ttggatgaga caaatgatga
1081gctggcccag atcgccaggg aaagagatat cttggctcat gacaatgaca atctccagga
1141acagtttgct aaagctaaac aagaaaacca ggcactgtcc aaaaaattga atgacactca
1201taatgaactt aatgacataa aacagaaggt tcaagatact aatttggagg ttaacaagct
1261gaagaatata ttaaagtctg aagaatctga gaaccggcaa atgatggaac aacttcgaaa
1321agccaatgaa gatgctgaaa actgggaaaa taaagcccgt caatcagagg cagataacaa
1381taccctcaaa ctggaactta tcactgctga ggcagagggt aacagattaa aagaaaaagt
1441agattccctc aacagagagg ttgagcaaca cttaaatgca gaaaggtctt acaagtccca
1501gatttctacc ttacataaat ctgttgtaaa aatggaagag gagcttcaga aggttcagtt
1561tgaaaaagtg tccgctcttg cagatttgtc ttctactagg gaactctgta ttaaacttga
1621ctcaagcaaa gaacttctta atcgacagct ggttgctaaa gatcaagaaa tagaaatgag
1681ggagaatgag ttagattctg ctcattctga aattgaactc ctgaggagtc agatggcaaa
1741tgagagaaTC TCCATGCAGA ATCTAGAAGC TTTGCTGGTG GCCAATCGAG ACAAAGAATA
1801TCAGTCTCAG ATAGCACTTC AAGAAAAAGA ATCTGAAATT CAGCTTCTTA AAGAACACCT
1861TTGTTTGGCA GAAAATAAAA TGGCCATCCA GAGTAGAGAT GTGGCCCAGT TCAGAAATGT
1921TGTCACACAA TTGGAAGCTG ATTTAGACAT TACCAAAAGA CAACTAGGAA CAGAGCGCTT
1981TGAAAGGGAG AGGGCCGTAC AAGAACTTCG CCGCCAAAAT TATTCAAGTA ATGCTTATCA
2041TATGAGTTCT ACAATGAAGC CAAATACAAA ATGTCATTCA CCAGAACGTG CTCACCATCG
2101ATCTCCTGAC CGAGGCCTAG ATCGATCATT AGAAGAGAAT CTTTGCTACA GAGATTTCTG
2161CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG
2221TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT
2281TTATATATCT TGTGGAAAGG ACGACCATCA GATCATGACC TAGCGTTCAC GCGTTAAGTC
2341gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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