Construct: ORF TRCN0000473691
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005047.1_s317c1
- Derived from:
- ccsbBroadEn_07940
- DNA Barcode:
- GGTTTAACTTACTTCCATGAGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NGEF (25791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473691
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25791 | NGEF | neuronal guanine nucleotide... | NM_019850.3 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 25791 | NGEF | neuronal guanine nucleotide... | XM_011510923.3 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 25791 | NGEF | neuronal guanine nucleotide... | NM_001114090.2 | 84% | 82.6% | (many diffs) |
| 4 | human | 25791 | NGEF | neuronal guanine nucleotide... | XM_011510924.1 | 60.8% | 60.9% | 0_1ins831;222T>C;240C>T |
| 5 | human | 25791 | NGEF | neuronal guanine nucleotide... | XM_011510925.1 | 60.8% | 60.9% | 0_1ins831;222T>C;240C>T |
| 6 | mouse | 53972 | Ngef | neuronal guanine nucleotide... | NM_001111314.1 | 83.6% | 86% | (many diffs) |
| 7 | mouse | 53972 | Ngef | neuronal guanine nucleotide... | NM_019867.2 | 73.9% | 79.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2196
- ORF length:
- 2130
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaccagggaa tctgaagatt tggaaaagac ccggaggaaa tcagcaagtg 121 atcaatggaa cactgataat gaaccagcca aggtgaaacc tgagttactc ccagaaaaag 181 aggagacttc tcaagctgac caggatatcc aagacaaaga gcctcattgc cacatcccaa 241 ttaagagaaa ttccatcttc aatcgctcca taagacgcaa aagcaaagcc aaggccagag 301 acaaccccga acggaacgcc agctgcctgg cagattcaca ggacaatgga aaatctgtaa 361 atgagcccct gaccttgaat atcccctgga gcagaacgcc tccttgcaga acagcaatgc 421 agacagaccc aggagcccag gaaatgagtg agtcgtcctc caccccggga aatggggcca 481 cgcccgagga gtggccggcc ctggccgaca gccccaccac gctcaccgag gccctgcgga 541 tgatccaccc cattcccgcc gactcctgga gaaacctcat tgaacaaata gggctcctgt 601 atcaggaata ccgagataaa tcgactctcc aagaaatcga aaccaggagg caacaggatg 661 cagaaataga agacaatacc aatgggtccc cggccagtga ggacaccccg gaggaggaag 721 aagaagagga ggaggaggag gagccggcca gcccaccaga gaggaagact ctgccccaga 781 tctgcctgct cagtaacccc cactcaaggt tcaacctctg gcaggatctt cccgagatcc 841 ggagcagcgg ggtgcttgag atcctacagc ctgaggagat taagctgcag gaggccatgt 901 tcgagctggt cacttccgag gcgtcctact acaagagtct gaacctgctc gtgtcccact 961 tcatggagaa cgagcggata aggaagatcc tgcacccgtc cgaggcgcac atcctcttct 1021 ccaacgtcct ggacgtgctg gctgtcagtg agcggttcct cctggagctg gagcaccgga 1081 tggaggagaa catcgtcatc tctgacgtgt gtgacatcgt gtaccgttat gcggctgacc 1141 acttctctgt ctacatcacc tacgtcagca atcagaccta ccaggagcgg acctataagc 1201 agctgctcca ggagaaggca gctttccggg agctgatcgc gcagctagag ctcgacccca 1261 agtgcagggg gctgcccttc tcctccttcc tcatcctgcc tttccagagg atcacacgcc 1321 tcaagctgtt ggtccagaac atcctgaaga gggtagaaga gaggtctgag cgggagtgca 1381 ctgctttgga tgctcacaag gagctggaaa tggtggtgaa ggcatgcaac gagggcgtca 1441 ggaaaatgag ccgcacggaa cagatgatca gcattcagaa gaagatggag ttcaagatca 1501 agtcggtgcc catcatctcc cactcccgct ggctgctgaa gcagggtgag ctgcagcaga 1561 tgtcaggccc caagacctcc cggaccctga ggaccaagaa gctcttccac gaaatttacc 1621 tcttcctgtt caacgacctg ctggtgatct gccggcagat tccaggagac aagtaccagg 1681 tatttgactc agctccgcgg ggactgctgc gtgtggagga gctggaggac cagggccaga 1741 cgctggccaa cgtgttcatc ctgcggctgc tggagaacgc agatgaccgg gaggccaccT 1801 ACATGCTAAA GGCGTCCTCT CAGAGTGAGA TGAAGCGTTG GATGACCTCA CTGGCCCCCA 1861 ACAGGAGGAC CAAGTTTGTT TCGTTCACAT CCCGGCTGCT GGACTGCCCC CAGGTCCAGT 1921 GCGTGCACCC ATACGTGGCT CAGCAGCCAG ACGAGCTGAC GCTGGAGCTC GCCGACATCC 1981 TCAACATCCT GGACAAGACT GACGACGGGT GGATCTTTGG CGAGCGTCTG CACGACCAGG 2041 AGAGAGGCTG GTTCCCCAGC TCCATGACTG AGGAGATCTT GAATCCCAAG ATCCGGTCCC 2101 AGAACCTCAA GGAATGTTTC CGTGTCCACA AGATGGATGA CCCTCAGCGC AGCCAGAACA 2161 AGGACCGCAG GAAGCTGGGC AGCCGGAATC GGCAATGCCC AACTTTCTTG TACAAAGTGG 2221 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2281 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2341 AGGTTTAACT TACTTCCATG AGTGGACGCG TTAAGTCgac aatcaacctc tggattacaa 2401 aatttgtgaa agatt