Transcript: Mouse NM_001111314.1

Mus musculus neuronal guanine nucleotide exchange factor (Ngef), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Ngef (53972)
Length:
3127
CDS:
257..2389

Additional Resources:

NCBI RefSeq record:
NM_001111314.1
NBCI Gene record:
Ngef (53972)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001111314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000193333 CTAAGTACTTAGGCAGTATTA pLKO.1 2599 3UTR 100% 13.200 18.480 N Ngef n/a
2 TRCN0000174393 CTATATCACTTACGTCAGTAA pLKO.1 1342 CDS 100% 4.950 6.930 N Ngef n/a
3 TRCN0000240855 TGACCGAGAGGCCACCTATAT pLKO_005 1975 CDS 100% 13.200 10.560 N Ngef n/a
4 TRCN0000215945 CATGGAGGAGAACATTGTTAT pLKO.1 1270 CDS 100% 13.200 9.240 N Ngef n/a
5 TRCN0000257135 CCTCTGAGGCCTCCTACTATA pLKO_005 1104 CDS 100% 13.200 9.240 N Ngef n/a
6 TRCN0000257122 GACGCTGGAACTGGCAGATAT pLKO_005 2149 CDS 100% 13.200 9.240 N Ngef n/a
7 TRCN0000240854 GGAATGTTTCCGGGTACATAA pLKO_005 2302 CDS 100% 13.200 9.240 N Ngef n/a
8 TRCN0000240856 CACGTGTCCTTCCCTACTTTG pLKO_005 2759 3UTR 100% 10.800 7.560 N Ngef n/a
9 TRCN0000047655 CCAGAACCTCAAGGAATGTTT pLKO.1 2290 CDS 100% 5.625 3.938 N NGEF n/a
10 TRCN0000176123 GAACAAATAGGGCTCCTGTAT pLKO.1 767 CDS 100% 4.950 3.465 N Ngef n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001111314.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07940 pDONR223 100% 83.6% 86% None (many diffs) n/a
2 ccsbBroad304_07940 pLX_304 0% 83.6% 86% V5 (many diffs) n/a
3 TRCN0000473691 GGTTTAACTTACTTCCATGAGTGG pLX_317 18.9% 83.6% 86% V5 (many diffs) n/a
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