Construct: ORF TRCN0000473712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010321.1_s317c1
- Derived from:
- ccsbBroadEn_06210
- DNA Barcode:
- GAGGGATGGAATACATTTCCAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF12 (2257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473712
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2257 | FGF12 | fibroblast growth factor 12 | NM_004113.6 | 99.6% | 99.4% | 24C>A;446C>A |
| 2 | human | 2257 | FGF12 | fibroblast growth factor 12 | XM_006713538.3 | 97.7% | 96.6% | 4_4delAinsGAGAGCAAAG;15C>A;437C>A |
| 3 | human | 2257 | FGF12 | fibroblast growth factor 12 | XM_005247227.2 | 86.6% | 85% | (many diffs) |
| 4 | human | 2257 | FGF12 | fibroblast growth factor 12 | XM_024453395.1 | 86.5% | 86.1% | 0_1ins72;374C>A |
| 5 | human | 2257 | FGF12 | fibroblast growth factor 12 | NM_021032.4 | 73.3% | 72.4% | (many diffs) |
| 6 | mouse | 14167 | Fgf12 | fibroblast growth factor 12 | NM_010199.4 | 92.6% | 99.4% | (many diffs) |
| 7 | mouse | 14167 | Fgf12 | fibroblast growth factor 12 | NM_001276419.2 | 80.5% | 85% | (many diffs) |
| 8 | mouse | 14167 | Fgf12 | fibroblast growth factor 12 | XM_006521793.3 | 79.7% | 86.1% | (many diffs) |
| 9 | mouse | 14167 | Fgf12 | fibroblast growth factor 12 | NM_183064.5 | 68.1% | 72.4% | (many diffs) |
| 10 | mouse | 14167 | Fgf12 | fibroblast growth factor 12 | NM_001276420.1 | 19.8% | 17.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGA GAGCAAAGAA CCCCAGCTAA AAGGGATTGT GACAAGGTTA TTCAGCCAGC 121 AGGGATACTT CCTGCAGATG CACCCAGATG GTACCATTGA TGGGACCAAG GACGAAAACA 181 GCGACTACAC TCTCTTCAAT CTAATTCCCG TGGGCCTGCG TGTAGTGGCC ATCCAAGGAG 241 TGAAGGCTAG CCTCTATGTG GCCATGAATG GTGAAGGCTA TCTCTACAGT TCAGATGTTT 301 TCACTCCAGA ATGCAAATTC AAGGAATCTG TGTTTGAAAA CTACTATGTG ATCTATTCTT 361 CCACACTGTA CCGCCAGCAA GAATCAGGCC GAGCTTGGTT TCTGGGACTC AATAAAGAAG 421 GTCAAATTAT GAAGGGGAAC AGAGTGAAGA AAACCAAGCC CTCATCACAT TTTGTACCGA 481 AACCTATTGA AGTGTGTATG TACAGAGAAC AATCGCTACA TGAAATTGGA GAAAAACAAG 541 GGCGTTCAAG GAAAAGTTCT GGAACACCAA CCATGAATGG AGGCAAAGTT GTGAATCAAG 601 ATTCAACATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGAGGGA TGGAATACAT TTCCAACGAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt