Construct: ORF TRCN0000473762
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009333.1_s317c1
- Derived from:
- ccsbBroadEn_14387
- DNA Barcode:
- CCATGGTATTATAGACGTCGGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCM2L (140706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473762
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140706 | CCM2L | CCM2 like scaffold protein | NM_001365692.1 | 26.7% | 26.6% | 1_1254del;1475A>G |
| 2 | human | 140706 | CCM2L | CCM2 like scaffold protein | XM_011528568.3 | 25.9% | 25.8% | 1_1230del;1378_1455del;1529A>G |
| 3 | human | 140706 | CCM2L | CCM2 like scaffold protein | XM_011528567.3 | 25.8% | 25.7% | 1_1233del;1381_1458del;1532A>G |
| 4 | human | 140706 | CCM2L | CCM2 like scaffold protein | XM_011528566.2 | 25.5% | 25.4% | 1_1254del;1402_1479del;1553A>G |
| 5 | mouse | 228788 | Ccm2l | cerebral cavernous malforma... | XM_011239482.1 | 31.7% | 32.3% | (many diffs) |
| 6 | mouse | 228788 | Ccm2l | cerebral cavernous malforma... | XM_006499326.2 | 22.6% | 22.9% | (many diffs) |
| 7 | mouse | 228788 | Ccm2l | cerebral cavernous malforma... | XM_006499325.2 | 22.5% | 22.9% | (many diffs) |
| 8 | mouse | 228788 | Ccm2l | cerebral cavernous malforma... | NM_145536.3 | 14.1% | 7% | (many diffs) |
| 9 | mouse | 228788 | Ccm2l | cerebral cavernous malforma... | XR_001782331.1 | 8.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAATAAG 61 TTGGCATGGT CACGTTGCGG AGTAAGCTGG GGCCCCTCGA GATCCAGCAG TTTGCGATGC 121 TGCTGCGGGA GTACCGGCTG GGGCTGCCCA TCCAGGACTA TTGCACAGGC CTGCTGAAGC 181 TCTACGGAGA CCGGCGCAAG TTCCTCCTCC TTGGGATGCG GCCCTTCATC CCGGACCAGG 241 ACATCGGCTA CTTCGAGGGC TTCCTGGAGG GCGTGGGCAT CCGCGGGGGC GGCATCCTCA 301 CTGACAGCTT CGGCCGCATC AAGCGCAGCA TGAGCTCCAC GTCGGCCTCC GCAGTGCGCA 361 GCTACGATGG CGCGGCGCAG CGGCCCGAGG CACAGGCCTT CCACCGGCTG CTGGCTGACA 421 TCACGCACGA CATCGAGGCG CTGGCCCCCG ATGACGACGA CGACGACGAG GATGAGCCCC 481 GGGGCTCCAG GGGCGGGAGC GACGCCGCAG AAGACAACTA CCTGTACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA CCATGGTATT ATAGACGTCG GTCTACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt