Construct: ORF TRCN0000473772

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014240.1_s317c1
Derived from:
ccsbBroadEn_12793
DNA Barcode:
TTGATGTTGGAGTCAAATGCATTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP48 (84196)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473772
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84196 USP48 ubiquitin specific peptidas... XM_024450167.1 40.9% 40.9% 1_1098del
2 human 84196 USP48 ubiquitin specific peptidas... XM_011542267.3 40.9% 40.9% 1_1101del
3 human 84196 USP48 ubiquitin specific peptidas... XM_011542268.2 40.9% 40.9% 1_1101del
4 human 84196 USP48 ubiquitin specific peptidas... XM_017002497.1 40.9% 40.9% 1_1101del
5 human 84196 USP48 ubiquitin specific peptidas... XM_011542265.3 27.9% 27.9% 1_1962del
6 human 84196 USP48 ubiquitin specific peptidas... NM_001350164.2 26.2% 26.2% 1_2142del
7 human 84196 USP48 ubiquitin specific peptidas... XM_011542264.1 26.2% 26.2% 1_2142del
8 human 84196 USP48 ubiquitin specific peptidas... XM_006710955.4 26.2% 26.2% 1_2145del
9 human 84196 USP48 ubiquitin specific peptidas... NM_001350166.2 24.5% 24.5% 1_2337del
10 human 84196 USP48 ubiquitin specific peptidas... NM_001350167.2 24.5% 24.5% 1_2340del
11 human 84196 USP48 ubiquitin specific peptidas... NM_001350168.2 24.5% 24.5% 1_2340del
12 human 84196 USP48 ubiquitin specific peptidas... NM_032236.8 24.5% 24.5% 1_2343del
13 human 84196 USP48 ubiquitin specific peptidas... XR_002957781.1 20.4% 1_2446del;3209_3726del
14 human 84196 USP48 ubiquitin specific peptidas... XR_001737480.2 19.7% 1_2558del;2599_2600insTATAG;3316_3833del
15 human 84196 USP48 ubiquitin specific peptidas... XR_001737479.2 19.7% 1_2561del;2602_2603insTATAG;3319_3836del
16 human 84196 USP48 ubiquitin specific peptidas... NM_001330394.2 19.5% 19.5% 1_2343del;2727_2728ins156
17 human 84196 USP48 ubiquitin specific peptidas... XR_001737482.2 7.2% (many diffs)
18 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320012.1 41.2% 45.7% (many diffs)
19 mouse 170707 Usp48 ubiquitin specific peptidas... XM_006538606.3 41.1% 45.6% (many diffs)
20 mouse 170707 Usp48 ubiquitin specific peptidas... XM_006538607.3 41.1% 45.6% (many diffs)
21 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320010.1 21% 23.3% (many diffs)
22 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320009.1 20.9% 23.2% (many diffs)
23 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320013.1 20.9% 23.2% (many diffs)
24 mouse 170707 Usp48 ubiquitin specific peptidas... NM_001347227.1 20.9% 23.2% (many diffs)
25 mouse 170707 Usp48 ubiquitin specific peptidas... NM_130879.2 20.6% 22.9% (many diffs)
26 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320006.1 20.6% 22.8% (many diffs)
27 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320007.1 20.6% 22.8% (many diffs)
28 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320005.1 20.6% 22.8% (many diffs)
29 mouse 170707 Usp48 ubiquitin specific peptidas... XM_017320011.1 17.8% 19.5% (many diffs)
30 mouse 170707 Usp48 ubiquitin specific peptidas... XR_001784100.1 11.2% (many diffs)
31 mouse 170707 Usp48 ubiquitin specific peptidas... XR_001784101.1 10.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
828
ORF length:
762
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61tttgcatgtt tacatttgct tccatgacca aagaagattc taaacttata gctctcatat
121ggcccagtga gtggcaaatg atacaaaagc tctttgttgt ggatcatgta attaaaatca
181cgagaattga agtgggagat gtaaaccctt cagaaacaca gtatatttct gagcccaaac
241tctgtccaga atgcagagaa ggcttattgt gtcagcagca gagggacctg cgtgaataca
301ctcaagccac catctatgtc cataAAGTTG TGGATAATAA AAAGGTGATG AAGGATTCGG
361CTCCGGAACT GAATGTGAGT AGTTCTGAAA CAGAGGAGGA CAAGGAAGAA GCTAAACCAG
421ATGGAGAAAA AGATCCAGAT TTTAATCAAA GCAATGGTGG AACAAAGCGG CAAAAGATAT
481CCCATCAAAA TTATATAGCC TATCAAAAGC AAGTTATTCG CCGAAGTATG CGACATAGAA
541AAGTTCGTGG TGAGAAAGCA CTTCTCGTTT CTGCTAATCA GACGTTAAAA GAATTGAAAA
601TTCAGATCAT GCATGCATTT TCAGTTGCTC CTTTTGACCA GAATTTGTCA ATTGATGGAA
661AGATTTTAAG TGATGACTGT GCCACCCTAG GCACCCTTGG CGTCATTCCT GAATCTGTCA
721TTTTATTGAA GGCTGATGAA CCAATTGCAG ATTATGCTGC AATGGATGAT GTCATGCAAG
781TTTGTATGCC AGAAGAAGGG TTTAAAGGTA CTGGTCTTCT TGGACATTAC CCAACTTTCT
841TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
901CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
961GTGGAAAGGA CGATTGATGT TGGAGTCAAA TGCATTTACG CGTTAAGTCg acaatcaacc
1021tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)