Construct: ORF TRCN0000473809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010043.1_s317c1
- Derived from:
- ccsbBroadEn_00541
- DNA Barcode:
- TCTCGTGATTGAAAGAGCAGATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFEMP1 (2202)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | NM_001039348.3 | 100% | 100% | |
| 2 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | NM_001039349.3 | 100% | 100% | |
| 3 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | XM_024452755.1 | 100% | 100% | |
| 4 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | XM_024452756.1 | 100% | 100% | |
| 5 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | XM_017003586.2 | 83.7% | 83.7% | 638_639ins240 |
| 6 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | XM_024452757.1 | 83.7% | 83.7% | 638_639ins240 |
| 7 | human | 2202 | EFEMP1 | EGF containing fibulin extr... | XM_005264205.4 | 66.2% | 66.2% | 1_390del;1028_1029ins240 |
| 8 | mouse | 216616 | Efemp1 | epidermal growth factor-con... | NM_146015.2 | 88.8% | 94.5% | (many diffs) |
| 9 | mouse | 216616 | Efemp1 | epidermal growth factor-con... | XM_006514660.1 | 88.8% | 94.5% | (many diffs) |
| 10 | mouse | 216616 | Efemp1 | epidermal growth factor-con... | XM_011243695.1 | 81.6% | 86.6% | (many diffs) |
| 11 | mouse | 216616 | Efemp1 | epidermal growth factor-con... | XM_011243696.1 | 65.9% | 70.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gaaagccctt ttcctaacta tgctgactct ggcgctggtc aagtcacagg 121 acaccgaaga aaccatcacg tacacgcaat gcactgacgg atatgagtgg gatcctgtga 181 gacagcaatg caaagatatt gatgaatgtg acattgtccc agacgcttgt aaaggtggaa 241 tgaagtgtgt caaccactat ggaggatacc tctgccttcc gaaaacagcc cagattattg 301 tcaataatga acagcctcag caggaaacac aaccagcaga aggaacctca ggggcaacca 361 ccggggttgt agctgccagc agcatggcaa ccagtggagt gttgcccggg ggtggttttg 421 tggccagtgc tgctgcagtc gcaggccctg aaatgcagac tggccgaaat aactttgtca 481 tccggcggaa cccagctgac cctcagcgca ttccctccaa cccttcccac cgtatccagt 541 gtgcagcagg ctacgagcaa agtgaacaca acgtgtgcca agacatagac gagtgcactg 601 cagggacgca caactgtaga gcagaccaag tgtgcatcaa tttacgggga tcctttgcat 661 gtcagtgccc tcctggatat cagaagcgag gggagcagtg cgtagacata gatgaatgta 721 ccatccctcc atattgccac caaagatgcg tgaatacacc aggctcattt tattgccagt 781 gcagtcctgg gtttcaattg gcagcaaaca actatacctg cgtagatata aatgaatgtg 841 atgccagcaa tcaatgtgct cagcagtgct acaacattct tggttcattc atctgtcagt 901 gcaatcaagg atatgagcta agcagtgaca ggctcaactg tgaagacatt gatgaatgca 961 gaacctcaag ctacctgtgt caatatcaat gtgtcaatga acctgggaaa ttctcatgta 1021 tgtgccccca gggataccaa gtggtgagaa gtagaacatg tcaagatata aatgagtgtg 1081 agaccacaaa tgaatgccgg gaggatgaaa tgtgttggaa ttatcatggc ggcttccgtt 1141 gttatccacg aaatccttgt caagatccct acattctaac accagagaac cgatgtgttt 1201 gcccagtctc aaatgccatg tgccgagaac tgcccCAGTC AATAGTCTAC AAATACATGA 1261 GCATCCGATC TGATAGGTCT GTGCCATCAG ACATCTTCCA GATACAGGCC ACAACTATTT 1321 ATGCCAACAC CATCAATACT TTTCGGATTA AATCTGGAAA TGAAAATGGA GAGTTCTACC 1381 TACGACAAAC AAGTCCTGTA AGTGCAATGC TTGTGCTCGT GAAGTCATTA TCAGGACCAA 1441 GAGAACATAT CGTGGACCTG GAGATGCTGA CAGTCAGCAG TATAGGGACC TTCCGCACAA 1501 GCTCTGTGTT AAGATTGACA ATAATAGTGG GGCCATTTTC ATTTTACCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA TCTCGTGATT GAAAGAGCAG ATGCACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt