Construct: ORF TRCN0000473823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005433.1_s317c1
- Derived from:
- ccsbBroadEn_00069
- DNA Barcode:
- TCTACCTAGATTGAAATAGACTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANXA4 (307)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 307 | ANXA4 | annexin A4 | NM_001153.5 | 100% | 100% | |
| 2 | human | 307 | ANXA4 | annexin A4 | NM_001320698.2 | 100% | 100% | |
| 3 | human | 307 | ANXA4 | annexin A4 | NM_001365496.2 | 100% | 100% | |
| 4 | human | 307 | ANXA4 | annexin A4 | XM_017003943.1 | 100% | 100% | |
| 5 | human | 307 | ANXA4 | annexin A4 | XM_024452834.1 | 100% | 100% | |
| 6 | human | 307 | ANXA4 | annexin A4 | XM_024452835.1 | 100% | 100% | |
| 7 | human | 307 | ANXA4 | annexin A4 | NM_001320700.2 | 93.1% | 93.1% | 238_239ins66 |
| 8 | human | 307 | ANXA4 | annexin A4 | NM_001320702.2 | 73.8% | 73.8% | 0_1ins252 |
| 9 | human | 307 | ANXA4 | annexin A4 | XM_017003944.1 | 73.8% | 73.8% | 0_1ins252 |
| 10 | human | 307 | ANXA4 | annexin A4 | XR_001738734.2 | 34.1% | 1_119del;597_1379del;1866_2817del | |
| 11 | mouse | 11746 | Anxa4 | annexin A4 | NM_001331120.1 | 85.9% | 91.5% | (many diffs) |
| 12 | mouse | 11746 | Anxa4 | annexin A4 | NM_013471.2 | 85.9% | 91.5% | (many diffs) |
| 13 | mouse | 11746 | Anxa4 | annexin A4 | XM_006505397.2 | 85.9% | 91.5% | (many diffs) |
| 14 | mouse | 11746 | Anxa4 | annexin A4 | XM_006505398.2 | 85.9% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catggcaacc aaaggaggta ctgtcaaagc tgcttcagga ttcaatgcca 121 tggaagatgc ccagaccctg aggaaggcca tgaaagggct cggcaccgat gaagacgcca 181 ttattagcgt ccttgcctac cgcaacaccg cccagcgcca ggagatcagg acagcctaca 241 agagcaccat cggcagggac ttgatagacg acctgaagtc agaactgagt ggcaacttcg 301 agcaggtgat tgtggggatg atgacgccca cggtgctgta tgacgtgcaa gagctgcgaa 361 gggccatgaa gggagccggc actgatgagg gctgcctaat tgagatcctg gcctcccgga 421 cccctgagga gatccggcgc ataagccaaa cctaccagca gcaatatgga cggagccttg 481 aagatgacat tcgctctgac acatcgttca tgttccagcg agtgctggtg tctctgTCAG 541 CTGGTGGGAG GGATGAAGGA AATTATCTGG ACGATGCTCT CGTGAGACAG GATGCCCAGG 601 ACCTGTATGA GGCTGGAGAG AAGAAATGGG GGACAGATGA GGTGAAATTT CTAACTGTTC 661 TCTGTTCCCG GAACCGAAAT CACCTGTTGC ATGTGTTTGA TGAATACAAA AGGATATCAC 721 AGAAGGATAT TGAACAGAGT ATTAAATCTG AAACATCTGG TAGCTTTGAA GATGCTCTGC 781 TGGCTATAGT AAAGTGCATG AGGAACAAAT CTGCATATTT TGCTGAAAAG CTCTATAAAT 841 CGATGAAGGG CTTGGGCACC GATGATAACA CCCTCATCAG AGTGATGGTT TCTCGAGCAG 901 AAATTGACAT GTTGGATATC CGGGCACACT TCAAGAGACT CTATGGAAAG TCTCTGTACT 961 CGTTCATCAA GGGTGACACA TCTGGAGACT ACAGGAAAGT ACTGCTTGTT CTCTGTGGAG 1021 GAGATGATTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCTACC TAGATTGAAA TAGACTAAAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt