Construct: ORF TRCN0000473847
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000802.1_s317c1
- Derived from:
- ccsbBroadEn_11445
- DNA Barcode:
- ACACTGCCCGTATTCTGTATTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDAC6 (10013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473847
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321230.2 | 100% | 100% | |
| 2 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321231.2 | 100% | 100% | |
| 3 | human | 10013 | HDAC6 | histone deacetylase 6 | NR_135592.2 | 30.1% | (many diffs) | |
| 4 | human | 10013 | HDAC6 | histone deacetylase 6 | XM_017029187.1 | 23.4% | 23.3% | 1_42del;480_1866delinsG |
| 5 | human | 10013 | HDAC6 | histone deacetylase 6 | NR_135593.2 | 20.3% | (many diffs) | |
| 6 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321226.2 | 11.9% | 11.9% | 438_3645delinsG |
| 7 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321227.2 | 11.9% | 11.9% | 438_3645delinsG |
| 8 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321228.2 | 11.9% | 11.9% | 438_3645delinsG |
| 9 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321229.1 | 11.9% | 11.9% | 438_3645delinsG |
| 10 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_006044.4 | 11.9% | 11.9% | 438_3645delinsG |
| 11 | human | 10013 | HDAC6 | histone deacetylase 6 | NM_001321225.2 | 11.8% | 11.7% | 1_42del;480_3687delinsG |
| 12 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_002958755.1 | 11.6% | 1_79del;517_3748delinsG | |
| 13 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_002958757.1 | 11.6% | 1_79del;517_3764delinsG | |
| 14 | human | 10013 | HDAC6 | histone deacetylase 6 | NR_135591.1 | 11.3% | 1_30del;468_3855delinsG | |
| 15 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_246712.2 | 11.2% | 1_79del;517_3898delinsG | |
| 16 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_001755632.2 | 11.1% | 1_106del;544_3925delinsG | |
| 17 | human | 10013 | HDAC6 | histone deacetylase 6 | XR_002958756.1 | 11% | 1_79del;517_3957delinsG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 504
- ORF length:
- 438
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ctcaaccggc caggattcca ccacaaccag gcagcgaaga agtaggcaga 121 acccccagtc gccccctcag gactccagtg tcacttcGAA GCGAAATATT AAAAAGGGAG 181 CCGTTCCCCG CTCTATCCCC AATCTAGCGG AGGTAAAGAA GAAAGGCAAA ATGAAGAAGC 241 TCGGCCAAGC AATGGAAGAA GACCTAATCG TGGGACTGCA AGGGATGGAT CTGAACCTTG 301 AGGCTGAAGC ACTGGCTGGC ACTGGCTTGG TGTTGGATGA GCAGTTAAAT GAATTCCATT 361 GCCTCTGGGA TGACAGCTTC CCGGAAGGCC CTGAGCGGCT CCATGCCATC AAGGAGCAAC 421 TGATCCAGGA GGGCCTCCTA GATCGCTGCG TGTCCTTTCA GGCCCGGTTT GCTGAAAAGG 481 AAGAGCTGAT GTTGGTTCAC AGGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CACTGCCCGT 661 ATTCTGTATT AAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att