Construct: ORF TRCN0000473853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017257.1_s317c1
- Derived from:
- ccsbBroadEn_00971
- DNA Barcode:
- AGATTTAGCGGCTTCATCCCCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAL (4118)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473853
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4118 | MAL | mal, T cell differentiation... | NM_002371.4 | 100% | 100% | |
| 2 | human | 4118 | MAL | mal, T cell differentiation... | NM_022438.2 | 72.5% | 72.5% | 259_260ins126 |
| 3 | human | 4118 | MAL | mal, T cell differentiation... | NM_022439.2 | 63.3% | 63.3% | 93_94ins168 |
| 4 | human | 4118 | MAL | mal, T cell differentiation... | NM_022440.2 | 35.9% | 35.9% | 93_94ins294 |
| 5 | mouse | 17153 | Mal | myelin and lymphocyte prote... | NM_010762.5 | 85.8% | 86.9% | (many diffs) |
| 6 | mouse | 17153 | Mal | myelin and lymphocyte prote... | NM_001171187.1 | 54.4% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aaCAAGTTTG TACAAAAAAG 61 TTGGCATGGC CCCCGCAGCG GCGACGGGGG GCAGCACCCT GCCCAGTGGC TTCTCGGTCT 121 TCACCACCTT GCCCGACTTG CTCTTCATCT TTGAGTTTAT CTTCGGGGGC CTGGTGTGGA 181 TCCTGGTGGC CTCCTCCCTG GTGCCCTGGC CCCTGGTCCA GGGCTGGGTG ATGTTCGTGT 241 CTGTGTTCTG CTTCGTGGCC ACCACCACCT TGATCATCCT GTACATAATT GGAGCCCACG 301 GTGGAGAGAC TTCCTGGGTC ACCTTGGACG CAGCCTACCA CTGCACCGCT GCCCTCTTTT 361 ACCTCAGCGC CTCAGTCCTG GAGGCCCTGG CCACCATCAC GATGCAAGAC GGCTTCACCT 421 ACAGGCACTA CCATGAAAAC ATTGCTGCCG TGGTGTTCTC CTACATAGCC ACTCTGCTCT 481 ACGTGGTCCA TGCGGTGTTC TCTTTAATCA GATGGAAGTC TTCATACCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA AGATTTAGCG GCTTCATCCC CAAAACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt