Construct: ORF TRCN0000473875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003011.1_s317c1
- Derived from:
- ccsbBroadEn_05251
- DNA Barcode:
- CCACTCATGAAATTTCGTGCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1AKMT1 (221143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221143 | EEF1AKMT1 | EEF1A lysine methyltransfer... | NM_001318939.2 | 100% | 100% | |
| 2 | human | 221143 | EEF1AKMT1 | EEF1A lysine methyltransfer... | NM_174928.2 | 100% | 100% | |
| 3 | human | 221143 | EEF1AKMT1 | EEF1A lysine methyltransfer... | XM_017020432.1 | 100% | 100% | |
| 4 | human | 221143 | EEF1AKMT1 | EEF1A lysine methyltransfer... | NR_134934.1 | 64.4% | 1_57del;200_201ins83;617_785del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgatttggaa gatgatgaga caccccagct ttctgcccat gccttagcag 121 ctctccagga attttatgct gagcaaaagc aacaaattga gccaggcgag gatgataaat 181 ataacattgg aataatagaa gagaattggc aactgagcca gttttggtat agtcaggaaa 241 ctgctctgca gctggcacag gaggcaattg cagctgtagg agaaggtggc agaatcgcat 301 gtgtgagtgc ccctagtgtt taCCAGAAAC TCAGAGAGCT GTGCAGAGAA AACTTTTCGA 361 TATACATCTT TGAATATGAC AAAAGATTTG CCATGTATGG AGAGGAGTTT ATTTTCTATG 421 ATTACAATAA TCCATTGGAC TTACCCGAAA GAATTGCTGC ACATAGTTTT GACATCGTAA 481 TAGCAGATCC TCCCTATCTT TCGGAGGAAT GTCTCAGAAA AACATCGGAA ACCGTCAAGT 541 ACCTGACGCG GGGCAAGATT CTGCTGTGCA CAGGTGCCAT CATGGAAGAA CAGGCAGCAG 601 AACTCCTTGG AGTGAAGATG TGCACGTTTG TTCCAAGACA CACCCGGAAC TTGGCAAATG 661 AGTTTCGCTG TTATGTGAAT TATGATTCTG GGCTGGACTG TGGGATCTGC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGACCACTCA TGAAATTTCG TGCTTTAACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt