Construct: ORF TRCN0000473895
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006122.1_s317c1
- Derived from:
- ccsbBroadEn_08406
- DNA Barcode:
- TTATTGCACGAAAATGGTTAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A20 (54716)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473895
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54716 | SLC6A20 | solute carrier family 6 mem... | NM_020208.4 | 99.8% | 100% | 417T>C;1134C>T |
| 2 | human | 54716 | SLC6A20 | solute carrier family 6 mem... | NM_022405.4 | 93.6% | 93.7% | 417T>C;578_579ins111;1023C>T |
| 3 | human | 54716 | SLC6A20 | solute carrier family 6 mem... | XM_011533847.2 | 83.1% | 83.2% | 0_1ins297;120T>C;837C>T |
| 4 | human | 54716 | SLC6A20 | solute carrier family 6 mem... | XM_011533848.2 | 53.3% | 53.2% | (many diffs) |
| 5 | mouse | 102680 | Slc6a20a | solute carrier family 6 (ne... | NM_139142.2 | 87.8% | 92% | (many diffs) |
| 6 | mouse | 22599 | Slc6a20b | solute carrier family 6 (ne... | NM_011731.3 | 79% | 81.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1845
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaaagcg cggccgctgt gggccaactc gctacagttc gtgttcgcct 121 gcatctcgta cgccgtgggc ctgggcaacg tgtggcgatt cccgtacctg tgccagatgt 181 acggcggagg tagtttcctg gtcccctaca tcatcatgct tatcgtggag ggaatgccgc 241 tcttgtacct ggaactggct gtggggcagc gcatgcggca gggcagcatc ggcgcctgga 301 ggaccatcag cccgtacctc agtggtgtcg gggtcgccag cgtggtggtc tctttcttcc 361 tctccatgta ctacaacgtc atcaacgcct gggccttctg gtacctcttc cactccttcc 421 aggatcccct gccgtggtct gtctgcccac tgaatggtaa ccacacgggc tacgatgagg 481 agtgcgagaa ggcgtcctcc acacagtact tctggtacag gaaaaccctc aatatctcgc 541 cgtccctcca ggagaacggg ggtgtgcagt gggagccggc gctgtgcctc ctcctggcct 601 ggctggtggt gtacctgtgc atcctgcgtg gcaccgagtc cactggcaag gtggtgtatt 661 tcacggcgtc actgccctat tgcgtgctca tcatctacct catcaggggc ctcacgctcc 721 acggagccac caatggcctc atgtacatgt tcactcccaa gatagagcag ctggccaacc 781 ccaaggcctg gatcaatgca gccacccaga tcttcttctc acttggcctg ggcttcggca 841 gcctgatcgc cttcgccagc tacaatgagc catccaacaa ctgccagaag cacgccatca 901 tcgtgtccct catcaacagc ttcacctcca tatttgccag cattgtcacc ttctccatct 961 atggcttcaa ggccaccttc aattatgaaa actgcttgaa gaaggtgagt ctgctgctga 1021 ccaacacttt tgaccttgaa gatggctttt tgacagccag caacctggag caggtgaagg 1081 gctacctcgc atctgcctac ccaagcaaat acagcgagat gttcccgcaa atcaaaaact 1141 gcagcttgga atcggagcta gacacggccg tccagggcac tggcctggca ttcatcgtct 1201 atacagaggc cattaaaaac atggaggtgt cccagctgtg gtcggtgctc tacttcttca 1261 tgctgctgat gctgggcatt gggagcatgc tggggaacac agcggccatc ctcacccctc 1321 tgacagacag caagatcatc tccagccacc tgcccaagga ggccatctca ggtctggtgt 1381 gccttgtcaa ctgtgccatt ggcatggtgt tcacgatgga ggctgggaac tactggtttg 1441 acatattcaa cgactacgcg gccacactgt cccTGCTGCT CATCGTGCTG GTGGAGACGA 1501 TTGCCGTGTG CTACGTGTAC GGGCTGAGGA GATTTGAAAG TGACCTTAAG GCCATGACCG 1561 GCCGAGCTGT GAGCTGGTAC TGGAAGGTGA TGTGGGCTGG CGTAAGCCCA CTGCTGATTG 1621 TCAGCCTCTT TGTCTTCTAC CTGAGCGACT ACATCCTCAC GGGGACCCTG AAGTATCAAG 1681 CCTGGGACGC CTCCCAGGGC CAGCTCGTGA CCAAAGATTA CCCGGCCTAT GCACTGGCTG 1741 TCATCGGGCT GCTTGTGGCC TCCTCCACCA TGTGCATCCC CCTGGCGGCC CTGGGGACTT 1801 TTGTTCAGCG TCGCCTCAAG AGGGGAGACG CAGACCCCGT GGCCTTGCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA TTATTGCACG AAAATGGTTA AAAGACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt