Transcript: Human NM_022405.4

Homo sapiens solute carrier family 6 member 20 (SLC6A20), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
SLC6A20 (54716)
Length:
5314
CDS:
118..1785

Additional Resources:

NCBI RefSeq record:
NM_022405.4
NBCI Gene record:
SLC6A20 (54716)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_022405.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000435926 GAACTACTGGTTTGACATATT pLKO_005 1365 CDS 100% 13.200 10.560 N SLC6A20 n/a
2 TRCN0000412422 GCACATTGCAAACTGAAATAT pLKO_005 2077 3UTR 100% 15.000 10.500 N SLC6A20 n/a
3 TRCN0000038358 CAACAGCTTCACCTCCATATT pLKO.1 852 CDS 100% 13.200 9.240 N SLC6A20 n/a
4 TRCN0000425712 GAAAGCCGGTCTCAAAGATAA pLKO_005 2020 3UTR 100% 13.200 9.240 N SLC6A20 n/a
5 TRCN0000038354 GAGATTTGAAAGTGACCTTAA pLKO.1 1467 CDS 100% 10.800 7.560 N SLC6A20 n/a
6 TRCN0000038357 CGAGATGTTCCCGCAAATCAA pLKO.1 1053 CDS 100% 5.625 3.938 N SLC6A20 n/a
7 TRCN0000038356 CATGTACTACAACGTCATCAA pLKO.1 414 CDS 100% 4.950 3.465 N SLC6A20 n/a
8 TRCN0000038355 CTGCTGATTGTCAGCCTCTTT pLKO.1 1549 CDS 100% 4.950 3.465 N SLC6A20 n/a
9 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2604 3UTR 100% 4.050 2.025 Y P3H4 n/a
10 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2604 3UTR 100% 4.050 2.025 Y ORAI2 n/a
11 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2604 3UTR 100% 4.050 2.025 Y P3H4 n/a
12 TRCN0000161892 GCCTGTAATTCCAGCTACTTA pLKO.1 2703 3UTR 100% 5.625 2.813 Y GPN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_022405.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08406 pDONR223 100% 93.6% 93.7% None 417T>C;578_579ins111;1023C>T n/a
2 ccsbBroad304_08406 pLX_304 0% 93.6% 93.7% V5 417T>C;578_579ins111;1023C>T n/a
3 TRCN0000473895 TTATTGCACGAAAATGGTTAAAAG pLX_317 21.2% 93.6% 93.7% V5 417T>C;578_579ins111;1023C>T n/a
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