Construct: ORF TRCN0000473943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018354.1_s317c1
- Derived from:
- ccsbBroadEn_01229
- DNA Barcode:
- CCCATGCGTATATCACGGGGATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMP22 (5376)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5376 | PMP22 | peripheral myelin protein 22 | NM_000304.4 | 100% | 100% | |
| 2 | human | 5376 | PMP22 | peripheral myelin protein 22 | NM_001281455.1 | 100% | 100% | |
| 3 | human | 5376 | PMP22 | peripheral myelin protein 22 | NM_001281456.2 | 100% | 100% | |
| 4 | human | 5376 | PMP22 | peripheral myelin protein 22 | NM_153321.3 | 100% | 100% | |
| 5 | human | 5376 | PMP22 | peripheral myelin protein 22 | NM_153322.3 | 100% | 100% | |
| 6 | human | 5376 | PMP22 | peripheral myelin protein 22 | NM_001330143.2 | 65.9% | 68.1% | (many diffs) |
| 7 | human | 5376 | PMP22 | peripheral myelin protein 22 | XM_024450806.1 | 65.9% | 68.1% | (many diffs) |
| 8 | human | 5376 | PMP22 | peripheral myelin protein 22 | NR_104017.2 | 25.7% | (many diffs) | |
| 9 | human | 5376 | PMP22 | peripheral myelin protein 22 | NR_104018.2 | 23.6% | (many diffs) | |
| 10 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_001302255.1 | 86.8% | 86.2% | (many diffs) |
| 11 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_001302257.1 | 86.8% | 86.2% | (many diffs) |
| 12 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_001302258.1 | 86.8% | 86.2% | (many diffs) |
| 13 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_001302259.1 | 86.8% | 86.2% | (many diffs) |
| 14 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_001302260.1 | 86.8% | 86.2% | (many diffs) |
| 15 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_008885.3 | 86.8% | 86.2% | (many diffs) |
| 16 | mouse | 18858 | Pmp22 | peripheral myelin protein 22 | NM_001302261.1 | 51.4% | 52.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cctcctgttg ctgagtatca tcgtcctcca cgtcgcggtg ctggtgctgc 121 tgttcgtctc cacgatcgtc agccaatgga tcgtgggcaa tggacacgca actgatctct 181 ggcagaactg tagcacctct tccTCAGGAA ATGTCCACCA CTGTTTCTCA TCATCACCAA 241 ACGAATGGCT GCAGTCTGTC CAGGCCACCA TGATCCTGTC GATCATCTTC AGCATTCTGT 301 CTCTGTTCCT GTTCTTCTGC CAACTCTTCA CCCTCACCAA GGGGGGCAGG TTTTACATCA 361 CTGGAATCTT CCAAATTCTT GCTGGTCTGT GCGTGATGAG TGCTGCGGCC ATCTACACGG 421 TGAGGCACCC GGAGTGGCAT CTCAACTCGG ATTACTCCTA CGGTTTCGCC TACATCCTGG 481 CCTGGGTGGC CTTCCCCCTG GCCCTTCTCA GCGGTGTCAT CTATGTGATC TTGCGGAAAC 541 GCGAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCATGCGT ATATCACGGG GATACACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt