Construct: ORF TRCN0000473946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005879.1_s317c1
- Derived from:
- ccsbBroadEn_00912
- DNA Barcode:
- GCCCCTGTGATTAGGGTCCGGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLC1 (3831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473946
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3831 | KLC1 | kinesin light chain 1 | NM_005552.4 | 100% | 100% | |
| 2 | human | 3831 | KLC1 | kinesin light chain 1 | NM_182923.3 | 97.2% | 95.1% | (many diffs) |
| 3 | human | 3831 | KLC1 | kinesin light chain 1 | NM_001130107.1 | 89.7% | 89.6% | (many diffs) |
| 4 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001025359.2 | 86.3% | 97.8% | (many diffs) |
| 5 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_011244001.2 | 86.3% | 97.8% | (many diffs) |
| 6 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_006515495.2 | 84.9% | 96.4% | (many diffs) |
| 7 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001025363.2 | 84.8% | 96.2% | (many diffs) |
| 8 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001025358.2 | 84.8% | 96.2% | (many diffs) |
| 9 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_006515492.2 | 84.5% | 94.9% | (many diffs) |
| 10 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001025362.2 | 84.5% | 92.3% | (many diffs) |
| 11 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_006515494.2 | 84.5% | 92.3% | (many diffs) |
| 12 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_006515499.2 | 83.4% | 94.8% | (many diffs) |
| 13 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_006515498.2 | 83.3% | 94.8% | (many diffs) |
| 14 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_008450.2 | 83.2% | 94.6% | (many diffs) |
| 15 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_017314964.1 | 83.1% | 93.4% | (many diffs) |
| 16 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_017314965.1 | 81.8% | 93.2% | (many diffs) |
| 17 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001025361.2 | 79.1% | 88.3% | (many diffs) |
| 18 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001025360.2 | 78.4% | 88.1% | (many diffs) |
| 19 | mouse | 16593 | Klc1 | kinesin light chain 1 | XM_017314963.1 | 77.8% | 86.9% | (many diffs) |
| 20 | mouse | 16593 | Klc1 | kinesin light chain 1 | NM_001081959.1 | 77.1% | 86.8% | (many diffs) |
| 21 | mouse | 16593 | Klc1 | kinesin light chain 1 | XR_001780416.1 | 21.8% | (many diffs) | |
| 22 | mouse | 16593 | Klc1 | kinesin light chain 1 | XR_001780417.1 | 21.7% | (many diffs) | |
| 23 | mouse | 16593 | Klc1 | kinesin light chain 1 | XR_001780413.1 | 19.8% | (many diffs) | |
| 24 | mouse | 16593 | Klc1 | kinesin light chain 1 | XR_001780415.1 | 19.8% | (many diffs) | |
| 25 | mouse | 16593 | Klc1 | kinesin light chain 1 | XR_001780414.1 | 19.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1746
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta tgacaacatg tccacaatgg tgtacataaa ggaagacaag ttggagaagc 121 ttacacagga tgaaattatt tctaagacaa agcaagtaat tcaggggctg gaagctttga 181 agaatgagca caattccatt ttacaaagtt tgctggagac actgaagtgt ttgaagaaag 241 atgatgaaag taatttggtg gaggagaaat caaacatgat ccggaagtca ctggagatgt 301 tggagctcgg cctgagtgag gcacaggtta tgatggcttt gtcaaatcac ctgaatgctg 361 tggagtccga gaagcagaaa ctgcgtgcgc aggttcgtcg tctgtgccag gagaatcagt 421 ggctacggga tgaactggcc aacacgcagc agaaactgca gaagagtgag cagtctgtgg 481 ctcaactgga ggaggagaag aagcatctgg agtttatgaa tcagctaaaa aaatatgatg 541 acgacatttc cccatccgag gacaaagaca ctgattctac caaagagcct ctggatgacc 601 ttttccccaa tgatgaagac gacccagggc aaggaatcca gcagcagcac agcagtgcag 661 ccgcggctgc ccagcagggc ggctacgaga tccccgcgcg gctgcggacg ctccacaacc 721 tggtgatcca gtacgcctcg caggggcgct acgaggtagc tgtgcccctc tgcaagcagg 781 ccctggagga cctggagaag acttcaggac acgaccaccc ggacgtggcc accatgctca 841 acatcctggc cttggtgtac agggatcaga ataaatacaa agatgcagct aacctactga 901 atgatgcctt ggctattcgt gagaaaactt tgggcaaaga tcatcctgcg gtggcggcga 961 ctttgaataa ccttgcagtc ctttatggta aaagagggaa gtacaaagaa gcagagccgt 1021 tgtgtaaaag agctctggaa atccgagaaa aggttttggg gaaggatcac cccgatgttg 1081 ccaagcagtt aaataacttg gccttactgt gccagaacca gggcaagtat gaagaagtag 1141 aatattatta tcaaagagcc ctcgagatct accagacaaa actgggacct gatgacccca 1201 acgtggctaa gacgaaaaat aacctggcat cctgctattt gaaacaagga aagttcaagc 1261 aagcagaaac actgtacaaa gagattctca ctcgtgcaca tgaaagggag tttggttctg 1321 tagatgatga aaataaaccc atctGGATGC ATGCTGAAGA AAGAGAAGAA TGCAAAGGAA 1381 AGCAAAAGGA TGGGACATCT TTTGGAGAGT ATGGCGGCTG GTACAAAGCC TGCAAAGTTG 1441 ATAGTCCAAC TGTTACAACC ACTCTAAAAA ACCTTGGGGC ACTTTACAGA CGTCAAGGCA 1501 AATTTGAAGC TGCAGAAACG TTAGAAGAAG CTGCTATGAG GTCTCGTAAA CAGGGTCTTG 1561 ACAATGTTCA CAAACAGAGG GTGGCAGAAG TGCTCAATGA CCCTGAGAAC ATGGAGAAGC 1621 GCAGGAGCCG TGAGAGCCTC AACGTGGACG TGGTCAAGTA CGAGAGTGGC CCTGACGGAG 1681 GGGAGGAAGT GAGTATGAGC GTAGAGTGGA ACGGGATGAG GAAAATGAAG CTCGGGCTGG 1741 TTAACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1801 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1861 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCCCTGTG ATTAGGGTCC GGGTGACGCG 1921 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt