Construct: ORF TRCN0000473948
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018385.1_s317c1
- Derived from:
- ccsbBroadEn_09707
- DNA Barcode:
- CCAGACTGTGGGTCATGGTATCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC2A12 (154091)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473948
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154091 | SLC2A12 | solute carrier family 2 mem... | NM_145176.3 | 99.9% | 100% | 1023C>T |
| 2 | human | 154091 | SLC2A12 | solute carrier family 2 mem... | XM_006715349.4 | 81.9% | 77.5% | (many diffs) |
| 3 | human | 154091 | SLC2A12 | solute carrier family 2 mem... | XM_017010311.2 | 80.5% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1917
- ORF length:
- 1851
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt acctgttgaa aacaccgagg gccccagtct gctgaaccag aaggggacag 121 ccgtggagac ggagggcagc ggcagccggc atcctccctg ggcgagaggc tgcggcatgt 181 ttaccttcct gtcatctgtc actgctgctg tcagtggcct cctggtgggt tatgaacttg 241 ggatcatctc tggggctctt cttcagatca aaaccttatt agccctgagc tgccatgagc 301 aggaaatggt tgtgagctcc ctcgtcattg gagccctcct tgcctcactc accggagggg 361 tcctgataga cagatatgga agaaggacag caatcatctt gtcatcctgc ctgcttggac 421 tcggaagctt agtcttgatc ctcagtttat cctacacggt tcttatagtg ggacgcattg 481 ccataggggt ctccatctcc ctctcttcca ttgccacttg tgtttacatc gcagagattg 541 ctcctcaaca cagaagaggc cttcttgtgt cactgaatga gctgatgatt gtcatcggca 601 ttctttctgc ctatatttca aattacgcat ttgccaatgt tttccatggc tggaagtaca 661 tgtttggtct tgtgattccc ttgggagttt tgcaagcaat tgcaatgtat tttcttcctc 721 caagccctcg gtttctggtg atgaaaggac aagagggagc tgctagcaag gttcttggaa 781 ggttaagagc actctcagat acaactgagg aactcactgt gatcaaatcc tccctgaaag 841 atgaatatca gtacagtttt tgggatctgt ttcgttcaaa agacaacatg cggacccgaa 901 taatgatagg actaacacta gtattttttg tacaaatcac tggccaacca aacatattgt 961 tctatgcatc aactgttttg aagtcagttg gatttcaaag caatgaggca gctagcctcg 1021 cctccactgg ggttggagtc gtcaaggtca ttagcaccat ccctgccact cttcttgtag 1081 accatgttgg cagcaaaaca ttcctctgca ttggctcctc tgtgatggca gcttcgttgg 1141 tgaccatggg catcgtaaat ctcaacatcc acatgaactt cacccatatc tgcagaagcc 1201 acaattctat caaccagtcc ttggatgagt ctgtgattta tggaccagga aacctgtcaa 1261 ccaacaacaa tactctcaga gaccacttca aagggatttc ttcccatagc agaagctcac 1321 tcatgcccct gagaaatgat gtggataaga gaggggagac gacctcagca tccttgctaa 1381 atgctggatt aagccacact gaataccaga tagtcacaga ccctggggac gtcccagctt 1441 ttttgaaatg gctgtcctta gccagcttgc ttgtttatgt tgctgctttt tcaattggtc 1501 taggaccaat gccctggctg gtgctcagcg agatctttcc tggtgggatc agaggacgag 1561 ccatggcttt aacttcTAGC ATGAACTGGG GCATCAATCT CCTCATCTCG CTGACATTTT 1621 TGACTGTAAC TGATCTTATT GGCCTGCCAT GGGTGTGCTT TATATATACA ATCATGAGTC 1681 TAGCATCCCT GCTTTTTGTT GTTATGTTTA TACCTGAGAC AAAGGGATGC TCTTTGGAAC 1741 AAATATCAAT GGAGCTAGCA AAAGTGAACT ATGTGAAAAA CAACATTTGT TTTATGAGTC 1801 ATCACCAAGA AGAATTAGTG CCAAAACAGC CTCAAAAAAG AAAACCCCAG GAGCAGCTCT 1861 TGGAGTGTAA CAAGCTGTGT GGTAGGGGCC AATCCAGGCA GCTTTCTCCA GAGACCTACC 1921 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1981 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2041 ATATATCTTG TGGAAAGGAC GACCAGACTG TGGGTCATGG TATCGCACGC GTTAAGTCga 2101 caatcaacct ctggattaca aaatttgtga aagatt