Construct: ORF TRCN0000473959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006303.1_s317c1
- Derived from:
- ccsbBroadEn_08114
- DNA Barcode:
- TCGTACCCCAACGACGCCCACATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCG3 (29106)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473959
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29106 | SCG3 | secretogranin III | NM_013243.4 | 99.9% | 99.7% | 500T>C |
| 2 | human | 29106 | SCG3 | secretogranin III | NM_001165257.1 | 50.4% | 50.4% | 0_1ins696 |
| 3 | mouse | 20255 | Scg3 | secretogranin III | NM_009130.3 | 85.7% | 88.1% | (many diffs) |
| 4 | mouse | 20255 | Scg3 | secretogranin III | NM_001164790.1 | 84.9% | 86.8% | (many diffs) |
| 5 | mouse | 20255 | Scg3 | secretogranin III | XM_006510881.3 | 79.5% | 81.9% | (many diffs) |
| 6 | mouse | 20255 | Scg3 | secretogranin III | XM_017313220.1 | 78.7% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gttcctcggg accggcactt ggattctggt gttagtgctc ccgattcaag 121 ctttccccaa acctggagga agccaagaca aatctctaca taatagagaa ttaagtgcag 181 aaagaccttt gaatgaacag attgctgaag cagaagaaga caagattaaa aaaacatatc 241 ctccagaaaa caagccaggt cagagcaact attcttttgt tgataacttg aacctgctaa 301 aggcaataac agaaaaggaa aaaattgaga aagaaagaca atctataaga agctccccac 361 ttgataataa gttgaatgtg gaagatgttg attcaaccaa gaatcgaaaa ctgatcgatg 421 attatgactc tactaagagt ggattggatc ataaatttca agatgatcca gatggtcttc 481 atcaactaga cgggactcct ttaaccgctg aagacattgt ccataaaatc gctgccagga 541 tttatgaaga aaatgacaga gccgcgtttg acaagattgt ttctaaacta cttaatctcg 601 gccttatcac agaaagccaa gcacatacac tggaagatga agtagcagag gttttacaaa 661 aattaatctc aaaggaagcc aacaattatg aggaggatcc caataagccc acaagctgga 721 ctgagaatca ggctggaaaa ataccagaga aagtgactcc aatggcagca attcaagatg 781 gtcttgctaa gggagaaaac gatgaaacag tatctaacac attaaccttg acaaatggct 841 tggaaaggag aactaaaacc tacagtgaag acaactttga ggaactccaa tatttcccaa 901 atttctatgc gcTACTGAAA AGTATTGATT CAGAAAAAGA AGCAAAAGAG AAAGAAACAC 961 TGATTACTAT CATGAAAACA CTGATTGACT TTGTGAAGAT GATGGTGAAA TATGGAACAA 1021 TATCTCCAGA AGAAGGTGTT TCCTACCTTG AAAACTTGGA TGAAATGATT GCTCTTCAGA 1081 CCAAAAACAA GCTAGAAAAA AATGCTACTG ACAATATAAG CAAGCTTTTC CCAGCACCAT 1141 CAGAGAAGAG TCATGAAGAA ACAGACAGTA CCAAGGAAGA AGCAGCTAAG ATGGAAAAGG 1201 AATATGGAAG CTTGAAGGAT TCCACAAAAG ATGATAACTC CAACCCAGGA GGAAAGACAG 1261 ATGAACCCAA AGGAAAAACA GAAGCCTATT TGGAAGCCAT CAGAAAAAAT ATTGAATGGT 1321 TGAAGAAACA TGACAAAAAG GGAAATAAAG AAGATTATGA CCTTTCAAAG ATGAGAGACT 1381 TCATCAATAA ACAAGCTGAT GCTTATGTGG AGAAAGGCAT CCTTGACAAG GAAGAAGCCG 1441 AGGCCATCAA GCGCATTTAT AGCAGCCTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCGTA 1621 CCCCAACGAC GCCCACATGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt