Transcript: Mouse XM_017313220.1

PREDICTED: Mus musculus secretogranin III (Scg3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Scg3 (20255)
Length:
8592
CDS:
6831..8132

Additional Resources:

NCBI RefSeq record:
XM_017313220.1
NBCI Gene record:
Scg3 (20255)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017313220.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426633 TAGAGCTCTCTTGCCATAAAT pLKO_005 8277 3UTR 100% 15.000 21.000 N Scg3 n/a
2 TRCN0000438437 ACCAACAAGCTGACGCTTATG pLKO_005 8047 CDS 100% 10.800 8.640 N Scg3 n/a
3 TRCN0000444564 AGAACGACAGAGGAGTGTTTG pLKO_005 7225 CDS 100% 10.800 7.560 N Scg3 n/a
4 TRCN0000217085 CAACTGGATGGAACTCCTTTA pLKO.1 7158 CDS 100% 10.800 7.560 N Scg3 n/a
5 TRCN0000215853 CCATCATGAAGACATTGATTG pLKO.1 7642 CDS 100% 10.800 7.560 N Scg3 n/a
6 TRCN0000425100 GAACGTGGAAGACGCTGATTC pLKO_005 7049 CDS 100% 10.800 7.560 N Scg3 n/a
7 TRCN0000189555 CCTTCCTGTTGTTCCAGCAAA pLKO.1 8152 3UTR 100% 4.950 3.465 N Scg3 n/a
8 TRCN0000190114 CTGACAAGCATCGACTCAGAA pLKO.1 7590 CDS 100% 4.950 3.465 N Scg3 n/a
9 TRCN0000191577 GAATCCTTATTTCCTCTTGAA pLKO.1 8345 3UTR 100% 4.950 3.465 N Scg3 n/a
10 TRCN0000191902 GCAATTATTCTTCTGTCGATA pLKO.1 6940 CDS 100% 4.950 3.465 N Scg3 n/a
11 TRCN0000190161 CCATAAGATTGCCACCAGGAT pLKO.1 7196 CDS 100% 2.640 1.848 N Scg3 n/a
12 TRCN0000190810 GATAACCAACTGAACGTGGAA pLKO.1 7038 CDS 100% 2.640 1.848 N Scg3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017313220.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08114 pDONR223 100% 78.7% 80.6% None (many diffs) n/a
2 ccsbBroad304_08114 pLX_304 0% 78.7% 80.6% V5 (many diffs) n/a
3 TRCN0000473959 TCGTACCCCAACGACGCCCACATG pLX_317 39.8% 78.7% 80.6% V5 (many diffs) n/a
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