Construct: ORF TRCN0000473960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002689.1_s317c1
- Derived from:
- ccsbBroadEn_11437
- DNA Barcode:
- ACAGGCCTCTTGCTCTGCATGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC23A2 (9962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9962 | SLC23A2 | solute carrier family 23 me... | NM_005116.6 | 64.5% | 64.3% | (many diffs) |
| 2 | human | 9962 | SLC23A2 | solute carrier family 23 me... | NM_203327.2 | 64.5% | 64.3% | (many diffs) |
| 3 | mouse | 54338 | Slc23a2 | solute carrier family 23 (n... | NM_018824.2 | 57% | 60.9% | (many diffs) |
| 4 | mouse | 54338 | Slc23a2 | solute carrier family 23 (n... | XM_006499906.3 | 57% | 60.9% | (many diffs) |
| 5 | mouse | 54338 | Slc23a2 | solute carrier family 23 (n... | XM_006499907.3 | 57% | 60.9% | (many diffs) |
| 6 | mouse | 54338 | Slc23a2 | solute carrier family 23 (n... | XM_011239698.2 | 57% | 60.9% | (many diffs) |
| 7 | mouse | 54338 | Slc23a2 | solute carrier family 23 (n... | XM_017319131.1 | 57% | 60.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1341
- ORF length:
- 1275
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gggtattggt aagaatacca catccaaatc aatggaggct ggaagttcaa 121 cagaaggcaa atacgaagac gaggcaaagc acccagcttt cttcactctt ccggtggtga 181 taaatggagg cgccacctcc agcggtgagc aggacaatga ggacactgag ctcatggcga 241 tctacactac ggaaaacggc attgcagaaa agagctctct cgctgagacc ctggatagca 301 ctggcagtct ggacccccag cgatcagaca tgatttatac catagaagat gttcctccct 361 ggtacctgtg tatatttctg gggctacagc actacctgac atgcttcagc ggcacgatcg 421 cagtgccctt cctgttggcc gatgccatgt gtgtggggta cgaccagtgg gccaccagcc 481 agctcattgg gaccattttc ttctgtgtgg gaatcactac tttgctacag acaacgtttg 541 gatgcaggtt acccctgttt caggccagtg cttttgcatt tttggcccct gctcgagcca 601 tcctgtcttt agataaatgg aaatgtaaca ccacagatgt ttcagttgcc aatggaacag 661 cagagctgtt gcacacagaa cacatctggt atccccggat ccgagagatc cagggggcca 721 tcatcatgtc ctcactgata gaagtagtca tcggcctcct cggcctgcct ggggctctac 781 tgaagtacat cggtcccttg accattacac ccacggtggc cctaattggc ctcTCTGGTT 841 TCCAGGCAGC GGGGGAGAGA GCCGGGAAGC ACTGGGGCAT TGCCATGCTG ACAATATTCC 901 TAGTATTACT GTTTTCTCAA TACGCCAGAA ATGTTAAATT TCCTCTCCCG ATTTATAAAT 961 CCAAGAAAGG ATGGACTGCG TACAAGTTAC AGCTGTTCAA AATGTTCCCT ATCATCCTGG 1021 CCATCCTGGT ATCCTGGCTG CTCTGCTTCA TCTTCACGGT GACAGACGTC TTCCCTCCCG 1081 ACAGCACAAA GTATGGCTTC TATGCTCGCA CAGATGCCAG GCAAGGCGTG CTTCTGGTAG 1141 CCCCGTGGTT TAAGGTTCCA TACCCATTTC AGTGGGGACT GCCCACCGTG TCTGCGGCCG 1201 GTGTCATCGG CATGCTCAGT GCCGTGGTCG CCAGCATCAT CGAGTCTATT GGTGACTACT 1261 ACGCCTGTGC ACGGCTGTCC TGTGCCCCAC CCCCCCCCAT CCACGCAATA AACAGGTACG 1321 TTCCTGAGAA GACAAGTTCC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1381 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1441 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAG GCCTCTTGCT 1501 CTGCATGTCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt