Construct: ORF TRCN0000473972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000126.1_s317c1
- Derived from:
- ccsbBroadEn_07106
- DNA Barcode:
- GGCACTCGGCTATTACTAGTGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTC4 (7268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473972
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7268 | TTC4 | tetratricopeptide repeat do... | NM_004623.5 | 99.9% | 99.7% | 139T>A |
| 2 | human | 7268 | TTC4 | tetratricopeptide repeat do... | NM_001291333.1 | 74.3% | 74.1% | 139T>A;679_680ins297 |
| 3 | human | 100527960 | MROH7-TTC4 | MROH7-TTC4 readthrough (NMD... | NR_037640.2 | 17.6% | (many diffs) | |
| 4 | human | 100527960 | MROH7-TTC4 | MROH7-TTC4 readthrough (NMD... | NR_037639.2 | 17.4% | (many diffs) | |
| 5 | human | 100527960 | MROH7-TTC4 | MROH7-TTC4 readthrough (NMD... | NR_037641.2 | 16.3% | (many diffs) | |
| 6 | mouse | 72354 | Ttc4 | tetratricopeptide repeat do... | NM_001172073.1 | 75% | 73.7% | (many diffs) |
| 7 | mouse | 72354 | Ttc4 | tetratricopeptide repeat do... | XM_006503409.3 | 65% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1227
- ORF length:
- 1161
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acaacctggg caggatccca cctcagacga cgtcatggac tcgttcctgg 121 aaaagttcca gagccagcct taccgtggcg gctttcatga ggaccagtgg gagaaggaat 181 ttgaaaaggt ccccctattt atgacgagag cgccatcaga aattgatccc agggagaatc 241 ctgacttggc ttgtctccag tcaattattt ttgatgagga gcgttctcca gaagaacagg 301 ccaagaccta taaagatgag ggcaatgatt actttaaaga aaaagactac aagaaagctg 361 taatttcata cactgaaggc ttaaagaaga aatgtgcaga tcctgatttg aatgctgtcc 421 tttataccaa ccgggcagca gcacagtact atctgggcaa ttttcgttct gctctcaatg 481 atgtgacagc tgccagaaag ctaaaaccct gccacctcaa agcaataata agaggtgcct 541 tatgccatct ggaactgaaa cactttgccg aggccgtgaa ctggtgtgat gagggactgc 601 aaatagatgc caaagagaag aagcttctgg aaatgagGGC TAAAGCAGAC AAGCTGAAGC 661 GAATTGAACA GAGGGATGTG AGGAAAGCCA ACTTGAAAGA AAAGAAGGAG AGGAATCAGA 721 ATGAGGCTTT ACTCCAGGCC ATCAAGGCTA GGAATATCAG GCTCTCAGAA GCTGCCTGTG 781 AGGATGAAGA TTCAGCCTCA GAAGGTCTAG GTGAGCTTTT CCTGGATGGA CTCAGCACTG 841 AGAACCCCCA TGGAGCCAGG CTGAGTCTAG ATGGCCAGGG CAGGCTGAGC TGGCCTGTGC 901 TCTTTCTGTA CCCAGAGTAT GCCCAGTCGG ACTTCATCTC TGCTTTTCAT GAGGACTCCA 961 GGTTTATTGA TCATCTAATG GTGATGTTTG GTGAAACACC CTCTTGGGAC CTAGAGCAAA 1021 AATATTGCCC TGATAATTTG GAGGTCTACT TTGAGGATGA GGACAGGGCA GAACTATACC 1081 GGGTGCCTGC CAAGAGCACC TTGCTACAGG TTCTACAGCA CCAGAGGTAC TTTGTAAAAG 1141 CCCTGACACC AGCATTTTTG GTCTGTGTAG GATCCTCTCC TTTTTGCAAG AATTTTCTCC 1201 GGGGGAGAAA GGTGTACCAG ATACGATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGCACTCG 1381 GCTATTACTA GTGACGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt