Construct: ORF TRCN0000473974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008847.1_s317c1
- Derived from:
- ccsbBroadEn_08982
- DNA Barcode:
- TACGTAGTTCTACTATCGCCCGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NAA60 (79903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001083600.3 | 99.8% | 100% | 552C>G |
| 2 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001083601.3 | 99.8% | 100% | 552C>G |
| 3 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_024845.4 | 99.8% | 100% | 552C>G |
| 4 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001317093.1 | 87.8% | 79.6% | (many diffs) |
| 5 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001317095.2 | 73% | 73.1% | 0_1ins195;357C>G |
| 6 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001317096.2 | 62.4% | 40.6% | (many diffs) |
| 7 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001317094.2 | 55.2% | 54.9% | 240_241ins229;244C>A;248_249ins95 |
| 8 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001317097.2 | 51.2% | 50.5% | 335_336ins298;429_537del |
| 9 | human | 79903 | NAA60 | N(alpha)-acetyltransferase ... | NM_001317098.2 | 51.2% | 50.5% | 335_336ins298;429_537del |
| 10 | mouse | 74763 | Naa60 | N(alpha)-acetyltransferase ... | NM_029090.3 | 93.1% | 97.1% | (many diffs) |
| 11 | mouse | 74763 | Naa60 | N(alpha)-acetyltransferase ... | XM_006522679.1 | 93.1% | 97.1% | (many diffs) |
| 12 | mouse | 74763 | Naa60 | N(alpha)-acetyltransferase ... | XM_006522680.2 | 93.1% | 97.1% | (many diffs) |
| 13 | mouse | 74763 | Naa60 | N(alpha)-acetyltransferase ... | NM_001290689.1 | 66.9% | 70.2% | (many diffs) |
| 14 | mouse | 74763 | Naa60 | N(alpha)-acetyltransferase ... | XM_011246038.2 | 66.9% | 70.2% | (many diffs) |
| 15 | mouse | 74763 | Naa60 | N(alpha)-acetyltransferase ... | XM_017317155.1 | 52% | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac agaggtggtg ccatccagcg cgctcagcga ggtcagcctg cgcctcctct 121 gccacgatga catagacact gtgaagcacc tgtgtggcga ctggttcccc atcgagtacc 181 cagactcatg gtatcgtgat aTCACATCCA ACAAGAAGTT CTTTTCCCTT GCTGCAACCT 241 ACAGAGGTGC CATTGTGGGA ATGATAGTAG CTGAAATTAA GAACAGGACC AAAATACATA 301 AAGAGGATGG AGATATTCTA GCATCCAACT TCTCTGTTGA CACACAAGTC GCGTACATCC 361 TAAGTCTGGG CGTCGTGAAA GAGTTCAGGA AGCACGGCAT AGGTTCCCTC TTACTTGAAA 421 GTTTAAAGGA TCACATATCA ACCACCGCCC AGGACCACTG CAAAGCCATT TACCTGCATG 481 TCCTCACCAC CAACAACACA GCAATAAACT TCTATGAAAA CAGAGACTTC AAGCAGCACC 541 ACTATCTCCC CTATTACTAC TCCATTCGAG GGGTCCTCAA AGATGGCTTC ACCTATGTCC 601 TCTACATCAA CGGCGGGCAC CCTCCCTGGA CGATTTTGGA CTACATCCAG CACCTGGGCT 661 CTGCACTAGC CAGCCTGAGC CCCTGCTCCA TTCCGCACAG AGTCTACCGC CAGGCCCACA 721 GCCTGCTCTG CAGCTTCCTG CCATGGTCGG GCATCTCTTC CAAGAGTGGC ATCGAGTACA 781 GCCGGACCAT GTGACCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAC GTAGTTCTAC TATCGCCCGT 961 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t