Construct: ORF TRCN0000473978
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010678.1_s317c1
- Derived from:
- ccsbBroadEn_02731
- DNA Barcode:
- GACTACGACTAAGTGGCATACCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LARP4B (23185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473978
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23185 | LARP4B | La ribonucleoprotein 4B | NM_015155.3 | 100% | 100% | |
| 2 | human | 23185 | LARP4B | La ribonucleoprotein 4B | NM_001351277.2 | 95.4% | 95.4% | 1929_2033del |
| 3 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_011519435.2 | 95.4% | 95.4% | 1929_2033del |
| 4 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015990.1 | 95.4% | 95.4% | 1929_2033del |
| 5 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_005252435.3 | 79.5% | 79.5% | 0_1ins369;1560_1664del |
| 6 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015994.1 | 76.1% | 76.1% | 0_1ins528 |
| 7 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015992.1 | 74.1% | 71.7% | (many diffs) |
| 8 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015993.1 | 72.7% | 72.7% | 0_1ins528;1401_1505del |
| 9 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015988.1 | 58.1% | 56.6% | (many diffs) |
| 10 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015987.1 | 56.5% | 55.1% | (many diffs) |
| 11 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XM_017015989.1 | 51.1% | 49.3% | (many diffs) |
| 12 | human | 23185 | LARP4B | La ribonucleoprotein 4B | XR_001747070.1 | 26.1% | (many diffs) | |
| 13 | mouse | 217980 | Larp4b | La ribonucleoprotein domain... | NM_172585.3 | 86.7% | 87.7% | (many diffs) |
| 14 | mouse | 217980 | Larp4b | La ribonucleoprotein domain... | XM_006516476.2 | 86.7% | 87.7% | (many diffs) |
| 15 | mouse | 217980 | Larp4b | La ribonucleoprotein domain... | XM_006516477.3 | 86.7% | 87.7% | (many diffs) |
| 16 | mouse | 217980 | Larp4b | La ribonucleoprotein domain... | NM_001311117.1 | 76.5% | 76.9% | (many diffs) |
| 17 | mouse | 217980 | Larp4b | La ribonucleoprotein domain... | XM_011244253.2 | 36.5% | 35.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2283
- ORF length:
- 2214
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacttctgat caggacgcta aggttgtggc tgaaccgcag acgcagagag 121 tccaggaggg caaggacagc gctcatctga tgaatggtcc tatatctcaa accacttctc 181 agacaagttc catcccacct ttgagtcagg taccagcaac taaggtttca gagctgaacc 241 ctaatgcaga agtgtggggg gctcctgtgt tacatctgga agcaagcagt gctgctgacg 301 gtgtgagtgc tgcatgggag gaggtggctg gccaccacgc agaccgtggc ccgcagggat 361 cggatgccaa tggtgatggt gaccagggcc atgagaatgc cgcattgcca gacccgcagg 421 agtcggaccc agcagacatg aacgctctcg ctctgggtcc ctcagaatat gactctctgc 481 ctgaaaatag cgagacagga ggaaatgagt ctcaaccaga cagccaggaa gacccccgag 541 aagtacttaa aaaaacattg gaattctgct tatctaggga gaaccttgct agtgacatgt 601 atcttatatc acagatggat agtgaccagt atgtgccaat cacaacggtg gctaacctcg 661 accacatcaa gaagctcagc actgatgtgg acttgattgt ggaagtgcta agatctttac 721 ctttagtcca agtggatgaa aagggagaaa aagtaaggcc aaatcaaaat cgctgcatag 781 taatattgcg tgaaatatct gaatctaccc ccgtggaaga agtagaagca ctatttaaag 841 gagataattt accaaaattt ataaactgtg aatttgcata taatgataat tggtttatta 901 catttgaaac agaagctgat gcacaacagg cttacaaata ccttcgagaa gaagtcaaaa 961 cttttcaagg aaaaccaatt aaggcacgga taaaagcaaa ggcaatagct ataaacacat 1021 ttttgccaaa gaatggattt agacccctgg acgtgagcct gtatgcccag cagcgctacg 1081 cgacgtcgtt ctacttccct cccatgtaca gcccccagca gcagttcccc ctgtacagcc 1141 tgatcactcc ccagacgtgg tcagcaacgc acagctatct tgacccaccc ttggtaactc 1201 catttccaaa tactggattt ataaatgggt ttacgtctcc agcgttcaag cctgcggcgt 1261 ctcctctgac ttctctcaga cagtatcctc ctcgaagcag gaatcctagt aaatctcatc 1321 tgcggcatgc gattcctagt gcagagaggg gacctgggtt attagaaagt ccttcaatat 1381 ttaacttcac tgcagatcga ttaattaatg gtgtccggag tccacaaaca aggcaagcag 1441 gtcaaactag aacacggatt caaaaccctt cagcatatgc caagagagag gctgggcctg 1501 ggcgtgtgga gccaggcagt ctcgaatcct ctcctggttt agggagggga aggaagaatt 1561 cctttggcta ccggaagaaa agggaggaga agtttacaag cagccagaca cagtctccaa 1621 cgccaccaaa gcctccgtcg ccaagcttcg agctggggct gtccagcttc cctccattac 1681 ctggagctgc cggcaatttg aagacagagg acttgtttga aaacaggcta tctagcttga 1741 taataggacc atccaaagaa aggaccctca gtgcagacgc aagcgtgaac acccttcctg 1801 tagtggtctc cagagagccc tcggtgccgg cttcttgtgc tgtatcagca acgtacgagc 1861 gatccccctc cccagctcat ttacccgatg atcccaaggt ggcggagaaa cagagggaaa 1921 cccacagtgt ggacagactt ccttCCGCCC TCACTGCGAC CGCGTGTAAA TCGGTGCAGG 1981 TGAACGGAGC CGCCACGGAA TTGCGAAAGC CCAGCTACGC AGAGATTTGT CAGAGAACGA 2041 GTAAAGAGCC TCCTTCTTCC CCATTGCAAC CCCAAAAAGA ACAAAAGCCA AACACTGTTG 2101 GTTGTGGGAA GGAGGAAAAG AAGCTGGCAG AGCCCGCAGA GAGATACCGG GAGCCCCCAG 2161 CCCTCAAGTC CACACCTGGA GCCCCCAGAG ACCAGAGGCG GCCGGCGGGG GGCCGGCCCT 2221 CGCCCTCGGC CATGGGGAAG CGTCTCAGCC GAGAGCAGAG CACTCCCCCC AAGTCTCCTC 2281 AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2341 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2401 GGCTTTATAT ATCTTGTGGA AAGGACGAGA CTACGACTAA GTGGCATACC GCACGCGTTA 2461 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt