Construct: ORF TRCN0000473994
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013468.1_s317c1
- Derived from:
- ccsbBroadEn_00338
- DNA Barcode:
- TTGCCTACAGGTCACGACGCCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNN1 (1264)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473994
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1264 | CNN1 | calponin 1 | NM_001299.6 | 100% | 100% | |
| 2 | human | 1264 | CNN1 | calponin 1 | XM_024451361.1 | 94% | 91.4% | (many diffs) |
| 3 | human | 1264 | CNN1 | calponin 1 | XM_024451362.1 | 94% | 91.4% | (many diffs) |
| 4 | human | 1264 | CNN1 | calponin 1 | NM_001308341.2 | 83.1% | 83.1% | 0_1ins150 |
| 5 | human | 1264 | CNN1 | calponin 1 | NM_001308342.2 | 83.1% | 83.1% | 0_1ins150 |
| 6 | human | 1264 | CNN1 | calponin 1 | XM_005259741.2 | 83.1% | 83.1% | 0_1ins150 |
| 7 | human | 1264 | CNN1 | calponin 1 | XM_017026289.1 | 80% | 75.6% | (many diffs) |
| 8 | mouse | 12797 | Cnn1 | calponin 1 | NM_009922.4 | 89% | 97.6% | (many diffs) |
| 9 | mouse | 12797 | Cnn1 | calponin 1 | XM_011242388.2 | 76.7% | 84.1% | (many diffs) |
| 10 | mouse | 12797 | Cnn1 | calponin 1 | XM_006509969.3 | 74% | 80.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctctgctcac ttcaaccgag gccctgccta cgggctgtca gccgaggtta 121 agaacaagct ggcccagaag tatgaccacc agcgggagca ggagctgaga gagtggatcg 181 agggggtgac aggccgtcgc atcggcaaca acttcatgga cggcctcaaa gatggcatca 241 ttctttgcga attcatcaat aagctgcagc caggctccgt gaagaagatc aatgagtcaa 301 cccaaaattg gcaccagctg gagaacatcg gcaacttcat caaggccatc accaagtatg 361 gggtgaagcc ccacgacatt tttgaggcca acgacctgtt tgagaacacc aaccatacac 421 aggtgcagtc cacccTCCTG GCTTTGGCCA GCATGGCGAA GACGAAAGGA AACAAGGTGA 481 ACGTGGGAGT GAAGTACGCA GAGAAGCAGG AGCGGAAATT CGAGCCGGGG AAGCTAAGAG 541 AAGGGCGGAA CATCATTGGG CTGCAGATGG GCACCAACAA GTTTGCCAGC CAGCAGGGCA 601 TGACGGCCTA TGGCACCCGG CGCCACCTCT ACGACCCCAA GCTGGGCACA GACCAGCCTC 661 TGGACCAGGC GACCATCAGC CTGCAGATGG GCACCAACAA AGGAGCCAGC CAGGCTGGCA 721 TGACTGCGCC AGGGACCAAG CGGCAGATCT TCGAGCCGGG GCTGGGCATG GAGCACTGCG 781 ACACGCTCAA TGTCAGCCTG CAGATGGGCA GCAACAAGGG CGCCTCGCAG CGGGGCATGA 841 CGGTGTATGG GCTGCCACGC CAGGTCTACG ACCCCAAGTA CTGTCTGACT CCCGAGTACC 901 CAGAGCTGGG TGAGCCCGCC CACAACCACC ACGCACACAA CTACTACAAT TCCGCCTACC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GATTGCCTAC AGGTCACGAC GCCCAGACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt