Construct: ORF TRCN0000473998
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004453.1_s317c1
- Derived from:
- ccsbBroadEn_12489
- DNA Barcode:
- CTTAAATCGATCTACCCCACAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNDC3B (64778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473998
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_017007064.2 | 9.3% | 8.9% | (many diffs) |
| 2 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_017007062.1 | 5.6% | 5.4% | (many diffs) |
| 3 | human | 64778 | FNDC3B | fibronectin type III domain... | NM_001135095.2 | 5.2% | 4.9% | (many diffs) |
| 4 | human | 64778 | FNDC3B | fibronectin type III domain... | NM_022763.4 | 5.2% | 4.9% | (many diffs) |
| 5 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_024453716.1 | 5.2% | 4.9% | (many diffs) |
| 6 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_024453717.1 | 5.2% | 4.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 223
- ORF end:
- 424
- ORF length:
- 201
- Sequence:
-
1 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC 61 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT 121 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG 181 GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGATGAT GACCGACCAA 241 ATCCCTCTGG AACTGCCACC ATTGCTGAAC GGAGAGGTAG CCATGATGCC CCACTTGGTG 301 AATGGAGATG CAGCTCAGCA GGTTATTCTC GTTCAAGTTA ATCCAGGTGA GACTTTCACA 361 ATAAGAGCAG AGGATGGAAC ACTTCAGTGC ATTCAAGATG AAGTGGTGAA GAGAGCCTGC 421 GATTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 481 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 541 TGGCTTTATA TATCTTGTGG AAAGGACGAC TTAAATCGAT CTACCCCACA GTCACGCGTT 601 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att