Construct: ORF TRCN0000474004
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002126.1_s317c1
- Derived from:
- ccsbBroadEn_00967
- DNA Barcode:
- TTCCCTTAAAATCATCCATACATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEA5 (4104)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474004
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100533997 | MAGEA10-MAGEA5 | MAGEA10-MAGEA5 readthrough | NM_001204811.3 | 100% | 100% | |
| 2 | human | 4104 | MAGEA5 | MAGE family member A5 | NM_021049.4 | 100% | 100% | |
| 3 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011548.1 | 34.5% | 30.5% | (many diffs) |
| 4 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011549.1 | 34.5% | 30.5% | (many diffs) |
| 5 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011550.1 | 34.5% | 30.5% | (many diffs) |
| 6 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_002362.4 | 34.5% | 30.5% | (many diffs) |
| 7 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274678.3 | 34.5% | 30.5% | (many diffs) |
| 8 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274679.4 | 34.5% | 30.5% | (many diffs) |
| 9 | human | 4102 | MAGEA3 | MAGE family member A3 | NM_005362.4 | 34.3% | 28.3% | (many diffs) |
| 10 | human | 4105 | MAGEA6 | MAGE family member A6 | NM_005363.3 | 34.3% | 28.3% | (many diffs) |
| 11 | human | 4105 | MAGEA6 | MAGE family member A6 | NM_175868.2 | 34.3% | 28.3% | (many diffs) |
| 12 | human | 4102 | MAGEA3 | MAGE family member A3 | XM_005274676.3 | 34.3% | 28.3% | (many diffs) |
| 13 | human | 4102 | MAGEA3 | MAGE family member A3 | XM_006724818.2 | 34.3% | 28.3% | (many diffs) |
| 14 | human | 4102 | MAGEA3 | MAGE family member A3 | XM_011531160.3 | 34.3% | 28.3% | (many diffs) |
| 15 | human | 4102 | MAGEA3 | MAGE family member A3 | XM_011531161.2 | 34.3% | 28.3% | (many diffs) |
| 16 | human | 4100 | MAGEA1 | MAGE family member A1 | NM_004988.5 | 31.7% | 26.5% | (many diffs) |
| 17 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274677.2 | 27.6% | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 441
- ORF length:
- 372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctcttgag cagaagagtc agcactgcaa gcctgaggaa ggccttgaca 121 cccaagaaga ggccctgggc ctggtgggtg tgcaggctgc cactactgag gagcaggagg 181 ctgtgtcctc ctcctctcct ctggtcccag gcaccctggg GGAGGTGCCT GCTGCTGGGT 241 CACCAGGTCC TCTCAAGAGT CCTCAGGGAG CCTCCGCCAT CCCCACTGCC ATCGATTTCA 301 CTCTATGGAG GCAATCCATT AAGGGCTCCA GCAACCAAGA AGAGGAGGGG CCAAGCACCT 361 CCCCTGACCC AGAGTCTGTG TTCCGAGCAG CACTCAGTAA GAAGGTGGCT GACTTGATTC 421 ATTTTCTGCT CCTCAAGTAT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTCC CTTAAAATCA 601 TCCATACATA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt