Construct: ORF TRCN0000474016
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015515.1_s317c1
- Derived from:
- ccsbBroadEn_07679
- DNA Barcode:
- CCGCTAGCAGTTTAACTAGCCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF274 (10782)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474016
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10782 | ZNF274 | zinc finger protein 274 | NM_133502.3 | 99.9% | 99.8% | 439G>A |
| 2 | human | 10782 | ZNF274 | zinc finger protein 274 | NM_016325.3 | 95% | 94.9% | 159_160ins96;343G>A |
| 3 | human | 10782 | ZNF274 | zinc finger protein 274 | NM_001278734.1 | 93.5% | 93.4% | 0_1ins126;313G>A |
| 4 | human | 10782 | ZNF274 | zinc finger protein 274 | NM_016324.3 | 83.8% | 83.7% | 0_1ins315;124G>A |
| 5 | human | 10782 | ZNF274 | zinc finger protein 274 | XM_011526327.1 | 83.8% | 83.7% | 0_1ins315;124G>A |
| 6 | human | 10782 | ZNF274 | zinc finger protein 274 | XM_017026174.1 | 83.8% | 83.7% | 0_1ins315;124G>A |
| 7 | human | 10782 | ZNF274 | zinc finger protein 274 | XR_001753589.2 | 63.8% | (many diffs) | |
| 8 | human | 10782 | ZNF274 | zinc finger protein 274 | XR_001753588.2 | 61.1% | (many diffs) | |
| 9 | human | 10782 | ZNF274 | zinc finger protein 274 | XM_011526328.2 | 51.7% | 51.7% | 0_1ins945 |
| 10 | human | 10782 | ZNF274 | zinc finger protein 274 | XM_017026175.1 | 45.2% | 39% | 439G>A;737_738ins113;888_889ins958 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2025
- ORF length:
- 1959
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccaggctt ccgacggcct ggtcctgtga accagtgacc tttgaagatg 121 taacactggg ttttaccccg gaagagtggg gactgctgga cctcaaacag aagtccctgt 181 acagggaagt gatgctggag aactacagga acctggtctc agtggaacat cagctttcca 241 aaccagatgt ggtatctcag ttagaggagg cagaagattt ctggccagtg gagagaggaa 301 ttcctcaaga caccattcca gagtatcctg agctccagct ggaccctaaa ttggatcctc 361 ttcctgctga gagtccccta atgaacattg aggttgttga ggtcctcaca ctgaaccagg 421 aggtggctgg tccccggaat gcccagatcc aggccctata tgctgaagat ggaagcctga 481 gtgcagatgc ccccagtgag cagatccaac agcagggcaa gcatccaggt gaccctgagg 541 ccgcgcgcca gaggttccgg cagttccgtt ataaggacat gacaggtccc cgggaggccc 601 tggaccagct ccgagagctg tgtcaccagt ggctacagcc taaggcacgc tccaaggagc 661 agatcctgga gctgctggtg ctggagcagt tcctaggtgc actgcctgtg aagctccgga 721 catgggtgga atcgcagcac ccagagaact gccaagaggt ggtggccctg gtagagggtg 781 tgacctggat gtctgaggag gaagtacttc ctgcaggaca acctgccgag ggcaccacct 841 gctgcctcga ggtcactgcc cagcaggagg agaagcagga ggatgcagcc atctgcccag 901 tgacagtgct ccctgaggag ccagtgacct tccaggatgt ggctgtggac ttcagccggg 961 aggagtgggg gctgctgggc ccgacacaga ggaccgagta ccgcgatgtg atgctggaga 1021 cctttgggca cctggtctct gtggggtggg agactacact ggaaaataaa gagttagctc 1081 caaattctga cattcctgag gaagaaccag cccccagcct gaaagtacaa gaatcctcaa 1141 gggattgtgc cttgtcctct acattagaag ataccttgca gggtggggtc caggaagtcc 1201 aagacacagt gttgaagcag atggagtctg ctcaggaaaa agaccttcct cagaagaagc 1261 actttgacaa ccgtgagtcc caggcaaaca gtggtgctct tgacacaaac caagtttcgc 1321 tccagaaaat tgacaaccct gagtcccagg caaacagtgg cgctcttgac acaaaccaag 1381 ttttgctcca caaaattcct cctagaaaac gattgcgcaa acgtgactca caagttaaaa 1441 gtatgaaaca taattcacgt gtaaaaattc atcagaagag ctgtgaaagg caaaaggcca 1501 aggaaggcaa tggttgtagg aaaaccttca gtcggagtac taaacagatt acgtttataa 1561 gaattcacaa ggggagccaa gtttgccgat gcagtgaatg tggtaaaata ttccggaacc 1621 caagatactt TTCTGTGCAT AAGAAAATCC ATACCGGAGA GAGGCCCTAT GTGTGTCAAG 1681 ACTGTGGGAA AGGATTTGTT CAGAGCTCTT CCCTCACACA GCATCAGAGA GTTCATTCTG 1741 GAGAGAGACC ATTTGAATGT CAGGAGTGTG GGAGGACCTT CAATGATCGC TCAGCCATCT 1801 CCCAGCACCT GAGGACTCAC ACTGGCGCTA AGCCCTACAA GTGTCAGGAC TGTGGAAAAG 1861 CCTTCCGCCA GAGCTCCCAC CTCATCAGAC ATCAGAGGAC TCACACCGGG GAGCGCCCAT 1921 ATGCATGCAA CAAATGTGGA AAGGCCTTCA CCCAGAGCTC ACACCTTATT GGGCACCAGA 1981 GAACCCACAA TAGGACAAAG CGAAAGAAGA AACAGCCTAC CTCATACCCA ACTTTCTTGT 2041 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2101 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2161 GAAAGGACGA CCGCTAGCAG TTTAACTAGC CATCACGCGT TAAGTCgaca atcaacctct 2221 ggattacaaa atttgtgaaa gatt