Transcript: Human NM_016325.3

Homo sapiens zinc finger protein 274 (ZNF274), transcript variant ZNF274a, mRNA.

Source:
NCBI, updated 2018-06-30
Taxon:
Homo sapiens (human)
Gene:
ZNF274 (10782)
Length:
2794
CDS:
500..2365

Additional Resources:

NCBI RefSeq record:
NM_016325.3
NBCI Gene record:
ZNF274 (10782)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_016325.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229702 ACCTTCAGTCGGAGTACTAAA pLKO_005 1862 CDS 100% 13.200 18.480 N ZNF274 n/a
2 TRCN0000229701 CAAACGTGACTCACAAGTTAA pLKO_005 1756 CDS 100% 13.200 18.480 N ZNF274 n/a
3 TRCN0000229700 TTCCGGCAGTTCCGTTATAAG pLKO_005 893 CDS 100% 13.200 18.480 N ZNF274 n/a
4 TRCN0000014855 CGCAAACGTGACTCACAAGTT pLKO.1 1754 CDS 100% 4.950 6.930 N ZNF274 n/a
5 TRCN0000014853 CCACAATAGGACAAAGCGAAA pLKO.1 2323 CDS 100% 4.050 5.670 N ZNF274 n/a
6 TRCN0000014856 GCCCATATGCATGCAACAAAT pLKO.1 2253 CDS 100% 0.000 0.000 N ZNF274 n/a
7 TRCN0000219005 TCTTGACACAAACCAAGTTTC pLKO_005 1636 CDS 100% 10.800 7.560 N ZNF274 n/a
8 TRCN0000014854 CCAGCCTGAAAGTACAAGAAT pLKO.1 1452 CDS 100% 5.625 3.938 N ZNF274 n/a
9 TRCN0000014857 CAGGCCCTATATGCTGAAGAT pLKO.1 788 CDS 100% 4.950 3.465 N ZNF274 n/a
10 TRCN0000229703 TGTTCACTCATTTAGTCATTA pLKO_005 2599 3UTR 100% 13.200 7.920 N ZNF274 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_016325.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07679 pDONR223 100% 95% 94.9% None 159_160ins96;343G>A n/a
2 ccsbBroad304_07679 pLX_304 0% 95% 94.9% V5 159_160ins96;343G>A n/a
3 TRCN0000474016 CCGCTAGCAGTTTAACTAGCCATC pLX_317 20.4% 95% 94.9% V5 159_160ins96;343G>A n/a
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