Construct: ORF TRCN0000474036

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016503.1_s317c1
Derived from:
ccsbBroadEn_08825
DNA Barcode:
ACCACTCGTTCGCAAAAAGCTTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LHPP (64077)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474036
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64077 LHPP phospholysine phosphohistid... NM_001167880.2 99.8% 99.5% 281A>G
2 human 64077 LHPP phospholysine phosphohistid... NM_001318332.2 98.4% 97.6% 281A>G;625_631delTTACTTTinsGCC;635_636delTA
3 human 64077 LHPP phospholysine phosphohistid... XM_011540058.3 95.4% 94.5% (many diffs)
4 human 64077 LHPP phospholysine phosphohistid... XM_017016511.1 84.7% 84.2% 281A>G;628_629delGT;632_741delinsG
5 human 64077 LHPP phospholysine phosphohistid... NM_001318331.2 84.2% 75.1% (many diffs)
6 human 64077 LHPP phospholysine phosphohistid... XM_024448122.1 78.1% 77.6% 281A>G;628_629delGT;632_804delinsG
7 human 64077 LHPP phospholysine phosphohistid... NM_022126.4 77.5% 77% 281A>G;628_629delGT;632_810delinsG
8 human 64077 LHPP phospholysine phosphohistid... XM_005270026.4 75% 74.5% 281A>G;628_629delGT;632_837delinsG
9 human 64077 LHPP phospholysine phosphohistid... XM_017016510.1 70.7% 70.2% 281A>G;628_629delGT;632_888delinsG
10 human 64077 LHPP phospholysine phosphohistid... XM_017016509.1 54.9% 54.5% 281A>G;628_629delGT;632_1143delinsG
11 human 64077 LHPP phospholysine phosphohistid... XR_001747177.2 15.7% (many diffs)
12 mouse 76429 Lhpp phospholysine phosphohistid... NM_029609.1 65.3% 68.5% (many diffs)
13 mouse 76429 Lhpp phospholysine phosphohistid... XM_006508297.1 30.6% 33.3% (many diffs)
14 mouse 76429 Lhpp phospholysine phosphohistid... XM_006508298.3 29.6% 31.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
696
ORF length:
630
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc accgtggggc aagcggctgg ctggcgtgcg cggggtgctg cttgacatct
121cgggcgtgct gtacgacagc ggcgcgggcg gcggcacggc catcgccggc tcggtggagg
181cggtggccag actgaagcgt tcccggctga aggtgaggtt ctgcaccaac gagtcgcaga
241agTCCCGGGC AGAGCTGGTG GGGCAGCTTC AGAGGCTGGG ATTTGACATC TCTGAGCAGG
301AGGTGACCGC CCCGGCACCA GCTGCCTGCC AGATCCTGAA GGAGCGAGGC CTGCGACCAT
361ACCTGCTCAT CCATGACGGA GTCCGCTCAG AATTTGATCA GATCGACACA TCCAACCCAA
421ACTGTGTGGT AATTGCAGAC GCAGGAGAAA GCTTTTCTTA TCAAAACATG AATAACGCCT
481TCCAGGTGCT CATGGAGCTG GAAAAACCTG TGCTCATATC ACTGGGAAAA GGGCGTTACT
541ACAAGGAGAC CTCTGGCCTG ATGCTGGACG TTGGTCCCTA CATGAAGGCG CTTGAGTATG
601CCTGTGGCAT CAAAGCCGAG GTGGTGGGGA AGCCTTCTCC TGAGTTTTTC AAGTCTGCCC
661TGCAAGCGAT AGGAGTGGAA GCCCACCAGG CCCAGTGCCC AACTTTCTTG TACAAAGTGG
721TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
781CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
841AACCACTCGT TCGCAAAAAG CTTTTACGCG TTAAGTCgac aatcaacctc tggattacaa
901aatttgtgaa agatt

Download FASTA (ORF) (Full)