Construct: ORF TRCN0000474052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011379.1_s317c1
- Derived from:
- ccsbBroadEn_15441
- DNA Barcode:
- ACATACCTGGAATATGGGTGGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HGF (3082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3082 | HGF | hepatocyte growth factor | NM_001010933.3 | 100% | 100% | |
| 2 | human | 3082 | HGF | hepatocyte growth factor | XM_017012098.1 | 100% | 100% | |
| 3 | human | 3082 | HGF | hepatocyte growth factor | NM_001010931.3 | 98.2% | 98.2% | 481_495del |
| 4 | human | 3082 | HGF | hepatocyte growth factor | XM_017012097.1 | 98.2% | 98.2% | 481_495del |
| 5 | human | 3082 | HGF | hepatocyte growth factor | NM_001010934.3 | 70.4% | 70% | (many diffs) |
| 6 | human | 3082 | HGF | hepatocyte growth factor | NM_001010932.3 | 39.3% | 39.1% | 851_852delCTinsA;857_2169del |
| 7 | human | 3082 | HGF | hepatocyte growth factor | XM_011516115.2 | 39.3% | 39.1% | 851_852delCTinsA;857_2169del |
| 8 | human | 3082 | HGF | hepatocyte growth factor | NM_000601.6 | 39.1% | 38.8% | 481_495del;866_867delCTinsA;872_2184del |
| 9 | human | 3082 | HGF | hepatocyte growth factor | XM_006715956.2 | 39.1% | 38.8% | 481_495del;866_867delCTinsA;872_2184del |
| 10 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289460.2 | 62% | 62.1% | (many diffs) |
| 11 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289461.1 | 35.1% | 35.5% | (many diffs) |
| 12 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289458.1 | 34.9% | 35.3% | (many diffs) |
| 13 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289459.1 | 34.9% | 35.3% | (many diffs) |
| 14 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_010427.5 | 34.9% | 35.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 921
- ORF length:
- 855
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggtgaccaaa ctcctgccag ccctgctgct gcagcatgtc ctcctgcatc 121 tcctcctgct ccccatcgcc atcccctatg cagagggaca aaggaaaaga agaaatacaa 181 ttcatgaatt caaaaaatca gcaaagacta ccctaatcaa aatagatcca gcactgaaga 241 taaaaaccaa aaaagtgaat actgcagacc aatgtgctaa tagatgtact aggaataaag 301 gacttccatt cacttgcaag gcttttgttt ttgataaagc aagaaaacaa tgcctctggt 361 tccccttcaa tagcatgtca agtggagtga aaaaagaatt tggccatgaa tttgacctct 421 atgaaaacaa agactacatt agaaactgca tcattggtaa aggacgcagc tacaagggaa 481 cagtatctat cactaagagt ggcatcaaat gtcagccctg gagttccatg ataccacacg 541 aacacagcta tcggggtaaa gacctacagg aaaactactg tcgaaatcct cgaggggaag 601 aagggggacc cTGGTGTTTC ACAAGCAATC CAGAGGTACG CTACGAAGTC TGTGACATTC 661 CTCAGTGTTC AGAAGTTGAA TGCATGACCT GCAATGGGGA GAGTTATCGA GGTCTCATGG 721 ATCATACAGA ATCAGGCAAG ATTTGTCAGC GCTGGGATCA TCAGACACCA CACCGGCACA 781 AATTCTTGCC TGAAAGATAT CCCGACAAGG GCTTTGATGA TAATTATTGC CGCAATCCCG 841 ATGGCCAGCC GAGGCCATGG TGCTATACTC TTGACCCTCA CACCCGCTGG GAGTACTGTG 901 CAATTAAAAC ATGCGAGACA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAT ACCTGGAATA 1081 TGGGTGGTCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt