Construct: ORF TRCN0000474078
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011954.1_s317c1
- Derived from:
- ccsbBroadEn_01680
- DNA Barcode:
- TAACCCGGGTCGACAGGCTACGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR2E1 (7101)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474078
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7101 | NR2E1 | nuclear receptor subfamily ... | NM_003269.5 | 100% | 100% | |
| 2 | human | 7101 | NR2E1 | nuclear receptor subfamily ... | NM_001286102.1 | 90.3% | 88.9% | (many diffs) |
| 3 | mouse | 21907 | Nr2e1 | nuclear receptor subfamily ... | NM_152229.2 | 91.8% | 98.9% | (many diffs) |
| 4 | mouse | 21907 | Nr2e1 | nuclear receptor subfamily ... | XM_006512701.2 | 90.5% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caagccagcc ggatcaacaa gccgcatttt agatatcccc tgcaaagtgt 121 gtggcgaccg cagctcgggg aagcactacg gggtctacgc ctgcgacggc tgctcaggtt 181 ttttcaaacg gagcatccga aggaatagga cctatgtctg caaatctgga aaccagggag 241 gctgtccggt ggacaagacg cacagaaacc agtgcagggc gtgtcggctg aagaagtgtt 301 tggaagtcaa catgaacaaa gacgccgtgc agcacgagcg ggggcctcgg acgtccacca 361 tccgcaagca agtggccctc tacttccgtg gacacaagga ggagaacggg gccgccgcgc 421 actttccctc ggcggcgctc cctgcgccgg ccttcttcac cgcggtcacg cagctggagc 481 cgcacggcct ggagctggcc gcggtgtcca ccactccaga gcggcagacc ctcgtgagcc 541 tggctcagcc cacgcccaag tacccccatg aagtgaatgg gaccccaatg tatctctatg 601 aagtggccac ggagtcggtg tgtgaatcag ctgccagact tctcttcatg agcatcaagt 661 gggctaagag tgtgccagcc ttctccacgc tgtctttgca agaccagctg atgcttttgg 721 aagatgcttg gagagaactg tttgttctag gaatagcaca atgggccatt ccggttgatg 781 ctaaCACTCT ACTGGCTGTA TCTGGCATGA ACGGTGACAA CACAGATTCC CAGAAGCTGA 841 ACAAGATCAT ATCTGAAATA CAGGCTTTAC AAGAGGTGGT GGCTCGATTT AGACAACTCC 901 GGTTAGATGC TACTGAATTT GCCTGTCTAA AATGCATCGT CACTTTCAAA GCCGTTCCTA 961 CACATAGTGG TTCTGAACTG AGAAGTTTCC GGAATGCTGC CGCCATTGCA GCCCTTCAAG 1021 ATGAGGCTCA GCTAACGCTC AACAGCTACA TCCATACCAG ATATCCCACT CAACCCTGTC 1081 GCTTTGGAAA ACTCCTGTTG CTTTTGCCAG CTTTACGTTC TATTAGCCCA TCAACTATAG 1141 AAGAAGTGTT TTTCAAAAAA ACCATCGGCA ATGTGCCAAT TACAAGACTG CTTTCAGATA 1201 TGTACAAATC CAGTGATATC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATAAC CCGGGTCGAC 1381 AGGCTACGGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt