Construct: ORF TRCN0000474098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000129.4_s317c1
- Derived from:
- ccsbBroadEn_15074
- DNA Barcode:
- CTGAGGCAGCGTCGGCTATTCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STK26 (51765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_001042452.2 | 38.7% | 32.4% | 335_785del;863_1062del |
| 2 | human | 51765 | STK26 | serine/threonine kinase 26 | NM_016542.4 | 30.9% | 27.6% | (many diffs) |
| 3 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_001313744.1 | 29.3% | 28.3% | (many diffs) |
| 4 | mouse | 70415 | Stk26 | serine/threonine kinase 26 | NM_133729.2 | 28.5% | 27.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccactcgccg gtggctgtcc aagtgCCTGG GATGCAGAAT AACATAGCTG 121 ATCCAGAAGA ACTGTTCACA AAATTAGAGC GCATTGGGAA AGGCTCATTT GGGGAAGTTT 181 TCAAAGGAAT TGATAACCGT ACCCAGCAAG TCGTTGCTAT TAAAATCATA GACCTTGAGG 241 AAGCCGAAGA TGAAATAGAA GACATTCAGC AAGAAATAAC TGTCTTGAGT CAATGTGACA 301 GCTCATATGT AACAAAATAC TATGGGTCAT ATTTAAAGGG GTCTAAATTA TGGATAATAA 361 TGGAATACCT GGGCGGTGGT TCAGCACTGG ATCTTCTTCG AGCTTTACCA CCGTACGAAA 421 GAAGCCTGAT CCAAAGAAAG TACAGAATGG GGCAGAGCAA GATCTTGTGC AAACCCTGCC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GACTGAGGCA GCGTCGGCTA TTCGCAACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt