Construct: ORF TRCN0000474107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013339.1_s317c1
- Derived from:
- ccsbBroadEn_07207
- DNA Barcode:
- CTGCGGTCCACTTGAGTATAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GDF5 (8200)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8200 | GDF5 | growth differentiation fact... | NM_000557.5 | 99.8% | 99.8% | 826G>T;1017A>G;1401G>T |
| 2 | human | 8200 | GDF5 | growth differentiation fact... | NM_001319138.2 | 99.8% | 99.8% | 826G>T;1017A>G;1401G>T |
| 3 | mouse | 14563 | Gdf5 | growth differentiation fact... | NM_008109.3 | 89.9% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1569
- ORF length:
- 1503
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag actccccaaa ctcctcactt tcttgctttg gtacctggct tggctggacc 121 tggaattcat ctgcactgtg ttgggtgccc ctgacttggg ccagagaccc caggggacca 181 ggccaggatt ggccaaagca gaggccaagg agaggccccc cctggcccgg aacgtcttca 241 ggccaggggg tcacagctat ggtggggggg ccaccaatgc caatgccagg gcaaagggag 301 gcaccgggca gacaggaggc ctgacacagc ccaagaagga tgaacccaaa aagctgcccc 361 ccagaccggg cggccctgaa cccaagccag gacaccctcc ccaaacaagg caggctacag 421 cccggactgt gaccccaaaa ggacagcttc ccggaggcaa ggcaccccca aaagcaggat 481 ctgtccccag ctccttcctg ctgaagaagg ccagggagcc cgggccccca cgagagccca 541 aggagccgtt tcgcccaccc cccatcacac cccacgagta catgctctcg ctgtacagga 601 cgctgtccga tgctgacaga aagggaggca acagcagcgt gaagttggag gctggcctgg 661 ccaacaccat caccagcttt attgacaaag ggcaagatga ccgaggtccc gtggtcagga 721 agcagaggta cgtgtttgac attagtgccc tggagaagga tgggctgctg ggggccgagc 781 tgcggatctt gcggaagaag ccctcggaca cggccaagcc agcggccccc ggaggcgggc 841 gggctgccca gctgaagctg tccagctgcc ccagcggccg gcagccggcc tccttgctgg 901 atgtgcgctc cgtgccaggc ctggacggat ctggctggga ggtgttcgac atctggaagc 961 tcttccgaaa ctttaagaac tcggcccagc tgtgcctgga gctggaggcc tgggaacggg 1021 gcagggccgt ggacctccgt ggcctgggct tcgaccgcgc cgcccggcag gtccacgaga 1081 aggccctgtt cctggtgttt ggccgcacca agaaacggga cctgttcttt aatgagatta 1141 aggcccgctc tggccaggac gataagaccg tgtatgagta cctgttcagc cagcggcgaa 1201 aacggcgggc cccactggcc actcgccagg gcaagcgacc cagcaagaac cttaaggctc 1261 gctgcagtcg gaaggcactg catgtcaact tcaaggacat gggctgggac gactggatca 1321 tcgcacccct tgagtacgag gctttccact gcgaggggct gtgcgagttc ccattgcgct 1381 cccacctgga gcccacgaat catgcagtca tccagaccct gatgaactcc atggaccccg 1441 agtccacacc acccacctgc tgtgttccca cgcggctgag tcccatcagc atcctcttca 1501 ttgactctgc caacaacgtg gtgtataagc agtatgagga catggtcgtg gagtcGTGTG 1561 GCTGCAGGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGCGG TCCACTTGAG TATAATGTAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt