Construct: ORF TRCN0000474129
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000830.1_s317c1
- Derived from:
- ccsbBroadEn_14268
- DNA Barcode:
- GACCGGATGACCACTGCCTTGGTG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKRIP1 (79706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474129
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79706 | PRKRIP1 | PRKR interacting protein 1 | NM_024653.4 | 99.6% | 98.9% | 316G>T;546delG |
| 2 | human | 100630923 | LOC100630923 | LOC100289561-PRKRIP1 readth... | NR_038967.1 | 16.7% | (many diffs) | |
| 3 | mouse | 66801 | Prkrip1 | Prkr interacting protein 1 ... | NM_025774.3 | 88.9% | 90.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tagcccagcc gcctcctcgg tgcgaccacc gaggcccaag aaagagccgc 121 agacgctcgt catccccaag aatgcggcgg aggagcagaa gctcaagctg gagcggcTCA 181 TGAAGAACCC GGACAAAGCA GTTCCAATTC CAGAGAAAAT GAGTGAATGG GCACCTCGAC 241 CTCCCCCAGA ATTTGTCCGA GATGTCATGG GTTCAAGTGC TGGGGCCGGC AGTGGAGAGT 301 TCCACGTGTA CAGACATCTG CGCCGGAGAG AATATCAGCG ACAGGACTAC ATGGATGCCA 361 TGGCTGAGAA GCAAAAATTG TATGCAGAGT TTCAGAAAAG ACTGGAAAAG AATAAAATTG 421 CTGCAGAGGA GCAGACCGCA AAGCGCCGGA AGAAGCGCCA GAAGTTAAAA GAGAAGAAAT 481 TACTGGCAAA GAAGATGAAA CTTGAACAGA AGAAACAAGA AGGACCCGGT CAGCCCAAGG 541 AGCAGGGGTC CAGCAGCTCT GCGGAGGCAT CTGGAACAGA GGAGGAGGAG GAAGTGCCCA 601 GTTTCACCAT GGGCGATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 661 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 721 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACCGGAT GACCACTGCC 781 TTGGTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt