Transcript: Human NM_024653.4

Homo sapiens PRKR interacting protein 1 (PRKRIP1), mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
PRKRIP1 (79706)
Length:
2159
CDS:
50..604

Additional Resources:

NCBI RefSeq record:
NM_024653.4
NBCI Gene record:
PRKRIP1 (79706)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_024653.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249224 TCATGAAGAACCCGGACAAAG pLKO_005 162 CDS 100% 10.800 7.560 N Prkrip1 n/a
2 TRCN0000376602 TCATGAAGAACCCGGACAAAG pLKO_005 162 CDS 100% 10.800 7.560 N PRKRIP1 n/a
3 TRCN0000189877 CTCATGAAGAACCCGGACAAA pLKO.1 161 CDS 100% 4.950 3.465 N Prkrip1 n/a
4 TRCN0000202080 CCGGACAAAGCAGTTCCAATT pLKO.1 173 CDS 100% 10.800 6.480 N Prkrip1 n/a
5 TRCN0000377448 CGGACAAAGCAGTTCCAATTC pLKO_005 174 CDS 100% 10.800 6.480 N PRKRIP1 n/a
6 TRCN0000263278 CCCGAGTTCCTGAGTCTATTT pLKO_005 1896 3UTR 100% 13.200 6.600 Y PRKRIP1 n/a
7 TRCN0000143382 GAAGAAGCGCCAGAAGTTAAA pLKO.1 433 CDS 100% 13.200 6.600 Y PRKRIP1 n/a
8 TRCN0000365091 GGAAGAAGCGCCAGAAGTTAA pLKO_005 432 CDS 100% 13.200 6.600 Y PRKRIP1 n/a
9 TRCN0000263279 TACTGGCAAAGAAGATGAAAC pLKO_005 465 CDS 100% 10.800 5.400 Y PRKRIP1 n/a
10 TRCN0000144665 GAAAGGAAACCCAGAAACATT pLKO.1 1971 3UTR 100% 5.625 2.813 Y PRKRIP1 n/a
11 TRCN0000377446 ATTGGATGCAGAGTTTCAGAA pLKO_005 361 CDS 100% 4.950 2.475 Y PRKRIP1 n/a
12 TRCN0000377447 CATGGATGCCATGGCTGAGAA pLKO_005 334 CDS 100% 4.950 2.475 Y PRKRIP1 n/a
13 TRCN0000122551 CCCAGAATTTGTCCGAGATGT pLKO.1 229 CDS 100% 4.950 2.475 Y PRKRIP1 n/a
14 TRCN0000141946 GAGTTCCACGTGTACAGACAT pLKO.1 281 CDS 100% 4.950 2.475 Y PRKRIP1 n/a
15 TRCN0000144666 GCCACATATTTGAACAGTGAT pLKO.1 862 3UTR 100% 4.950 2.475 Y PRKRIP1 n/a
16 TRCN0000263277 TATCAGCGACAGGACTACATG pLKO_005 317 CDS 100% 4.950 2.475 Y PRKRIP1 n/a
17 TRCN0000141202 CCAGAATTTGTCCGAGATGTC pLKO.1 230 CDS 100% 4.050 2.025 Y PRKRIP1 n/a
18 TRCN0000144963 GAACAGAAGAAACAAGAAGGA pLKO.1 488 CDS 100% 2.640 1.320 Y PRKRIP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_024653.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14268 pDONR223 100% 99.6% 98.9% None 316G>T;546delG n/a
2 ccsbBroad304_14268 pLX_304 0% 99.6% 98.9% V5 (not translated due to frame shift) 316G>T;546delG n/a
3 TRCN0000474129 GACCGGATGACCACTGCCTTGGTG pLX_317 78.8% 99.6% 98.9% V5 (not translated due to frame shift) 316G>T;546delG n/a
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