Construct: ORF TRCN0000474132
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003173.1_s317c1
- Derived from:
- ccsbBroadEn_02729
- DNA Barcode:
- AATAATAGACAAATGTTTAACCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFTN1 (23180)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474132
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | NM_015150.2 | 100% | 100% | |
| 2 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_005264985.1 | 100% | 100% | |
| 3 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_005264986.2 | 100% | 100% | |
| 4 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_006713069.1 | 100% | 100% | |
| 5 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_011533530.1 | 100% | 100% | |
| 6 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_011533529.1 | 87.7% | 86.7% | (many diffs) |
| 7 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_017006007.2 | 73.5% | 62.1% | 1030_1031ins116;1275_1276ins343 |
| 8 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_017006005.1 | 71.5% | 67.9% | (many diffs) |
| 9 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_011533531.1 | 63.5% | 51.6% | (many diffs) |
| 10 | human | 23180 | RFTN1 | raftlin, lipid raft linker 1 | XM_017006006.2 | 61.5% | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1803
- ORF length:
- 1734
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggttgcgga ttgaacaagt tagagaaacg tgatgaaaaa cggcctggga 121 atatttattc aactttgaag aggcctcagg tggaaaccaa gatagatgtg tcctatgaat 181 accgcttcct ggagttcacg actctgagtg ctgcggagct ccctgggtcc tcagcagtga 241 ggctggcctc cctgcgtgac ctgcccgccc agctcctgga gctgtaccag cagggcttct 301 cgctggcggc cctgcacccc ttcgtgcagc ccacccatga gcgggagaag acgcccctgg 361 agcacatctt tagagccatc ctgatcaaga aaaccgacag atctcagaaa actgatcttc 421 acaatgaagg ctacatcttg gaattagatt gctgttcctc cttagaccac ccgacagacc 481 agaaactcat cccagagttc attaagaaga tccaggaggc tgcaagccag ggcctgaaat 541 tcgttggtgt tatacctcag taccattcct ctgtgaactc ggcaggcagc agtgctccgg 601 tgtctactgc caacagcacc gaggatgcca gagatgcaaa aaacgcacgt ggggatcacg 661 cgtcactgga gaatgagaaa ccggggactg gggatgtgtg cagtgctccg gctgggagaa 721 accaaagccc agagcccagc tcaggcccca gaggggaggt gcccctcgcc aagcagccca 781 gctcaccctc cggagaggga gatggtggag aactttcacc acagggggtg agcaagacac 841 tggatggacc ggagagcaac cccttggagg tgcatgaaga gccactctca gggaaaatgg 901 agatcttcac ccttttcaac aaaccgaaga gccatcagaa gtgccggcaa tactaccctg 961 tcaccattcc tctccatgtc tccaagaatg gccagacagt gagcggtttg gacgccaact 1021 ggttagagca catgagcgac cacttccgga aaggaggcat gctggtgaac gcagtcttct 1081 accttggaat agtgaatgat tccttacatg gcttgacaga tggagtattc atctttgaag 1141 ctgtttccac agaagatagc aaaaccatac agggctatga tgctattgtg gttgaacaat 1201 ggacagtcct ggaaggtgtc gaagtgcaga cagactacgt gcccctgctg aactcgctgg 1261 cggcctatgg ctggcagctc acctgtgtgc taccaactCC CGTCGTCAAG ACTACCAGCG 1321 AGGGGAGTGT ATCCACCAAG CAGATTGTCT TTCTTCAGAG ACCTTGTCTA CCTCAGAAAA 1381 TCAAGAAGAA GGAATCGAAG TTTCAGTGGC GATTCTCCAG AGAAGAAATG CACAACAGGC 1441 AGATGAGGAA ATCAAAAGGT AAACTCAGTG CCAGAGACAA ACAACAAGCA GAAGAAAATG 1501 AGAAGAACTT AGAAGACCAG TCTTCCAAAG CTGGAGACAT GGGAAACTGT GTTTCAGGAC 1561 AGCAGCAGGA GGGTGGAGTC TCCGAGGAGA TGAAGGGCCC TGTCCAAGAG GACAAGGGAG 1621 AACAGCTGTC CCCTGGTGGC CTGCTGTGTG GGGTGGGTGT GGAGGGTGAG GCTGTGCAGA 1681 ATGGTCCTGC CAGCCACAGC AGGGCCCTGG TGGGGATTTG CACTGGGCAC TCCAATCCTG 1741 GAGAGGATGC CAGGGACGGG GATGCTGAGG AAGTCAGAGA GCTTGGTACG GTTGAAGAAA 1801 ACTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TAATAGACAA ATGTTTAACC GGACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt