Construct: ORF TRCN0000474186
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016058.1_s317c1
- Derived from:
- ccsbBroadEn_05756
- DNA Barcode:
- TGTATAACCACATTTATCGGGCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACAA1 (30)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474186
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | NM_001130410.1 | 99.8% | 99.6% | 881T>C |
| 2 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | XM_006713122.1 | 90.8% | 90.6% | 445_543del;980T>C |
| 3 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | NM_001607.4 | 77.9% | 77.8% | 445_543del;817_996del;1160T>C |
| 4 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | XM_011533650.2 | 53% | 46.7% | (many diffs) |
| 5 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | XM_006713123.2 | 51.7% | 46.5% | (many diffs) |
| 6 | human | 30 | ACAA1 | acetyl-CoA acyltransferase 1 | NR_024024.1 | 49.9% | (many diffs) | |
| 7 | mouse | 235674 | Acaa1b | acetyl-Coenzyme A acyltrans... | NM_146230.3 | 66.1% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1059
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaggctgcag gtagtgctgg gccacctgag gggtccggcc gattccggct 121 ggatgccgca ggccgcgcct tgcctgagcg gtgccccgca ggcctcggcc gcggacgtgg 181 tggtggtgca cgggcggcgc acggccatct gccgggcggg ccgcggcggc ttcaaggaca 241 ccacccccga cgagcttctc tcggcagtca tgaccgcggt tctcaaggac gtgaatctga 301 ggccggaaca gctgggggac atctgtgtcg gaaatgtgct gcagcctggg gccggggcaa 361 tcatggcccg aatcgcccag tttctgagtg acatcccgga gactgtgcct ttgtccactg 421 tcaatagaca gtgttcgtcg gggctacagg cagtggccag catagcaggt ggcatcagaa 481 atgggtctta tgacattggc atggcctgtg ggataacctc tgagaatgtg gctgagcggt 541 ttggCATTTC ACGGGAGAAG CAGGATACCT TTGCCCTGGC TTCCCAGCAG AAGGCAGCAA 601 GAGCCCAGAG CAAGGGCTGT TTCCAAGCTG AGATTGTGCC TGTGACCACC ACGGTCCATG 661 ATGACAAGGG CACCAAGAGG AGCATCACTG TGACCCAGGA TGAGGGTATC CGCCCCAGCA 721 CCACCATGGA GGGCCTGGCC AAACTGAAGC CTGCCTTCAA GAAAGATGGT TCTACCACAG 781 CTGGGCTGAC AGTGAGTGAC GTGGACATCT TCGAGATCAA TGAGGCCTTT GCAAGCCAGG 841 CTGCCTACTG TGTGGAGAAG CTACGACTCC CCCCTGAGAA GGTGAACCCC CTGGGGGGTG 901 CAGTGGCCTT AGGGCACCCA CTGGGCTGCA CTGGGGCACG ACAGGCCATC ACGCTGCTCA 961 ATGAGCTGAA GCGCCGTGGG AAGAGGGCAT ACGGAGTGGT GTCCATGTGC ATCGGGACTG 1021 GAATGGGAGC CGCTGCCGTC TTTGAATACC CTGGGAACTG CCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGATGTATA ACCACATTTA TCGGGCTCAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt