Construct: ORF TRCN0000474190
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013998.3_s317c1
- Derived from:
- ccsbBroadEn_15149
- DNA Barcode:
- GTAATTAAAGATTTTCAGTGGGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FN3KRP (79672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474190
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79672 | FN3KRP | fructosamine 3 kinase relat... | NM_024619.4 | 99.8% | 100% | 12C>G |
| 2 | human | 79672 | FN3KRP | fructosamine 3 kinase relat... | XM_024450948.1 | 61.4% | 61.4% | 0_1ins357 |
| 3 | human | 79672 | FN3KRP | fructosamine 3 kinase relat... | NR_046408.2 | 47.4% | (many diffs) | |
| 4 | mouse | 238024 | Fn3krp | fructosamine 3 kinase relat... | NM_181420.3 | 85.3% | 88.9% | (many diffs) |
| 5 | mouse | 238024 | Fn3krp | fructosamine 3 kinase relat... | XM_006533274.1 | 80.8% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagctgctg aggcgcgagc tgggctgcag ctctgtcagg gccacgggcc 121 actcgggggg cgggtgcatc agccagggcc ggagctacga cacggatcaa ggacgagtgt 181 tcgtgaaagt gaaccccaag gcggaggcca gaagaatgtt tgaaggtgag atggcaagtt 241 taactgccat cctgaaaaca aacacggtga aagtgcccaa gcccatcaag gttctggatg 301 ccccaggcgg cgggagcgtg ctggtgatgg agcacatgga catgaggcat ctgagcagtc 361 atgctgcaaa gcttggagcc cagctggccg atttacacct tgataacaag aagcttggag 421 agatgcgcct gaaggaggcg ggcacagtgg ggagaggagg tgggcaggag gaacggccct 481 ttgtggcccg gtttggattt gacgtggtga cgtgctgtgg atacctcccc caggtgaatg 541 actggcagga ggactgggtc gtgttctatg cccggcagcg catTCAGCCC CAGATGGACA 601 TGGTGGAGAA GGAGTCTGGG GACAGGGAGG CCCTCCAGCT TTGGTCTGCT CTGCAGTTAA 661 AGATCCCTGA CCTGTTCCGT GACCTGGAGA TCATCCCAGC CTTACTCCAC GGGGACCTCT 721 GGGGTGGAAA CGTAGCAGAG GATTCCTCTG GGCCGGTGAT TTTTGACCCA GCTTCTTTCT 781 ACGGCCACTC GGAATATGAG CTGGCAATAG CTGGCATGTT TGGGGGCTTT AGCAGCTCCT 841 TTTACTCCGC CTACCACGGC AAAATCCCCA AGGCCCCAGG ATTCGAGAAG CGCCTTCAGT 901 TGTATCAGCT CTTTCACTAC TTGAACCACT GGAATCATTT TGGATCGGGG TACAGAGGAT 961 CCTCCCTGAA CATCATGAGG AATCTGGTCA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 1141 AATTAAAGAT TTTCAGTGGG GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1201 ttgtgaaaga tt