Construct: ORF TRCN0000474197
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016243.1_s317c1
- Derived from:
- ccsbBroadEn_01859
- DNA Barcode:
- TGACCAAGTCCCTTATTCCACGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTP4A2 (8073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474197
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | NM_001369859.1 | 100% | 100% | |
| 2 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | NM_001369860.1 | 100% | 100% | |
| 3 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | NM_080391.4 | 100% | 100% | |
| 4 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | NM_001195100.2 | 85% | 85% | 320_321ins75 |
| 5 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | NM_001195101.2 | 81.4% | 81.4% | 96_97ins93 |
| 6 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | NM_001369858.1 | 81.4% | 81.4% | 96_97ins93 |
| 7 | human | 8073 | PTP4A2 | protein tyrosine phosphatas... | XR_002957649.1 | 16.9% | 1_102del;604_2951del | |
| 8 | mouse | 19244 | Ptp4a2 | protein tyrosine phosphatas... | NM_001164745.1 | 95.4% | 100% | (many diffs) |
| 9 | mouse | 19244 | Ptp4a2 | protein tyrosine phosphatas... | NM_008974.4 | 95.4% | 100% | (many diffs) |
| 10 | mouse | 19244 | Ptp4a2 | protein tyrosine phosphatas... | XM_017320045.1 | 95.4% | 100% | (many diffs) |
| 11 | mouse | 19244 | Ptp4a2 | protein tyrosine phosphatas... | XM_006502863.3 | 82% | 85% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 567
- ORF length:
- 501
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccgtccagcc cctgtggaga tctcctatga gaacatgcgt tttcTGATAA 121 CTCACAACCC TACCAATGCT ACTCTCAACA AGTTCACAGA GGAACTTAAG AAGTATGGAG 181 TGACGACTTT GGTTCGAGTT TGTGATGCTA CATATGATAA AGCTCCAGTT GAAAAAGAAG 241 GAATCCACGT TCTAGATTGG CCATTTGATG ATGGAGCTCC ACCCCCTAAT CAGATAGTAG 301 ATGATTGGTT AAACCTGTTA AAAACCAAAT TTCGTGAAGA GCCAGGTTGC TGTGTTGCAG 361 TGCATTGTGT TGCAGGATTG GGAAGGGCAC CTGTGCTGGT TGCACTTGCT TTGATTGAAT 421 GTGGAATGAA GTACGAAGAT GCAGTTCAGT TTATAAGACA AAAAAGAAGG GGAGCGTTCA 481 ATTCCAAACA GCTGCTTTAT TTGGAGAAAT ACCGACCTAA GATGCGATTA CGCTTCAGAG 541 ATACCAATGG GCATTGCTGT GTTCAGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 601 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 661 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGACCAAG 721 TCCCTTATTC CACGCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 781 aagatt