Construct: ORF TRCN0000474198
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002403.1_s317c1
- Derived from:
- ccsbBroadEn_04116
- DNA Barcode:
- ACGACCAATATGTACTAAATTCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LIN28A (79727)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474198
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79727 | LIN28A | lin-28 homolog A | NM_024674.6 | 99.8% | 33% | 200_201insC |
| 2 | human | 79727 | LIN28A | lin-28 homolog A | XM_011542148.2 | 99.8% | 33% | 200_201insC |
| 3 | mouse | 83557 | Lin28a | lin-28 homolog A (C. elegans) | NM_145833.1 | 90.9% | 32% | (many diffs) |
| 4 | mouse | 83557 | Lin28a | lin-28 homolog A (C. elegans) | XM_011250361.2 | 69.2% | 9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 297
- ORF length:
- 231
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctccgtgtcc aaccagcagt ttgcaggtgg ctgcgccaag gcggcagaag 121 aggcgcccga ggaggcgccg gaggacgcgg cccgggcggc ggacgagcct cagctgctgc 181 acggtgcggg catctgtaag tggttcaacg tgcgcatggg gttcGGCTTC CTGTCCATGA 241 CCGCCCGCGC CGGGGTCGCG CTCGACCCCC CCAGTGGATG TCTTTGTGCA CCAGAGTAAG 301 CTGCACATGG AAGGGTTCCG GAGCTTGAAG GAGGGTGAGG CAGTGGAGTT CACCTTTAAG 361 AAGTCAGCCA AGGGTCTGGA ATCCATCCGT GTCACCGGAC CTGGTGGAGT ATTCTGTATT 421 GGGAGTGAGA GGCGGCCAAA AGGAAAGAGC ATGCAGAAGC GCAGATCAAA AGGAGACAGG 481 TGCTACAACT GTGGAGGTCT AGATCATCAT GCCAAGGAAT GCAAGCTGCC ACCCCAGCCC 541 AAGAAGTGCC ACTTCTGCCA GAGCATCAGC CATATGGTAG CCTCATGTCC GCTGAAGGCC 601 CAGCAGGGCC CTAGTGCACA GGGAAAGCCA ACCTACTTTC GAGAGGAAGA AGAAGAAATC 661 CACAGCCCTA CCCTGCTCCC GGAGGCACAG AATTGCCCAA CTTTCTTGTA CAAAGTGGTT 721 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 781 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA 841 CGACCAATAT GTACTAAATT CTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 901 tttgtgaaag att