Construct: ORF TRCN0000474200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015313.1_s317c1
- Derived from:
- ccsbBroadEn_15686
- DNA Barcode:
- TGCGCAGACGAGACGCCTTTAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP15 (9958)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474200
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9958 | USP15 | ubiquitin specific peptidas... | NM_001252079.1 | 100% | 100% | |
| 2 | human | 9958 | USP15 | ubiquitin specific peptidas... | NM_001351161.2 | 51% | 49.3% | (many diffs) |
| 3 | human | 9958 | USP15 | ubiquitin specific peptidas... | NM_001351162.1 | 33.9% | 32.3% | (many diffs) |
| 4 | human | 9958 | USP15 | ubiquitin specific peptidas... | NM_006313.3 | 24.2% | 23.9% | (many diffs) |
| 5 | human | 9958 | USP15 | ubiquitin specific peptidas... | NM_001252078.2 | 23.4% | 23.3% | (many diffs) |
| 6 | human | 9958 | USP15 | ubiquitin specific peptidas... | NM_001351159.2 | 11.1% | 11% | (many diffs) |
| 7 | human | 102465133 | MIR6125 | microRNA 6125 | NR_106740.1 | 5.2% | 1_56del;96_97ins665 | |
| 8 | human | 9958 | USP15 | ubiquitin specific peptidas... | NR_147081.2 | 4.6% | (many diffs) | |
| 9 | human | 9958 | USP15 | ubiquitin specific peptidas... | NR_147082.2 | 4.6% | (many diffs) | |
| 10 | human | 9958 | USP15 | ubiquitin specific peptidas... | NR_147078.2 | 4.6% | (many diffs) | |
| 11 | human | 9958 | USP15 | ubiquitin specific peptidas... | NR_147079.2 | 4.6% | (many diffs) | |
| 12 | human | 9958 | USP15 | ubiquitin specific peptidas... | NR_147080.2 | 4.5% | (many diffs) | |
| 13 | mouse | 14479 | Usp15 | ubiquitin specific peptidas... | XM_006513228.3 | 77.3% | 83.4% | (many diffs) |
| 14 | mouse | 14479 | Usp15 | ubiquitin specific peptidas... | XR_380365.3 | 56.5% | (many diffs) | |
| 15 | mouse | 14479 | Usp15 | ubiquitin specific peptidas... | NM_001301628.1 | 22.4% | 23.7% | (many diffs) |
| 16 | mouse | 14479 | Usp15 | ubiquitin specific peptidas... | NM_027604.4 | 21.6% | 23.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaaggcgga gcggcggatc tggacaccca gcggtctgac atcgcgacgc 121 tgctcaaaac ctcgctccgg aaaggggaca cctggtacct agtcgatagt cgctggttca 181 aacagtggaa aaaatatgtt ggctttgaca gttgggacaa ataccagatg ggagatcaaa 241 atgtgtatcc tggacccatt gataactctg gacttctcaa agatggtgat gcccagtcac 301 ttaaggaaca ccTTATTGAT GAATTGGATT ACATACTGTT GCCAACTGAA GGTTGGAATA 361 AACTTGTCAG CTGGTACACA TTGATGGAAG GTCAAGAGCC AATAGCACGA AAGGTGGTTG 421 AACAGGGTAT GTTTGTAAAG CACTGCAAAG TAGAAGTATA TCTCACAGAA TTGAAGCTAT 481 GTGAAAATGG AAACATGAAT AATGTTGTAA CTCGAAGATT TAGCAAAGCT GACACAATAG 541 ATACAATTGA AAAGGAAATA AGAAAAATCT TCAGTATTCC AGATGAAAAG GAGACCAGAT 601 TGTGGAACAA ATACATGAGT AACACATTTG AACCACTGAA TAAACCAGAC AGCACCATTC 661 AGGATGCTGG TTTATACCAA GGACAGGTAT TAGTGATAGA ACAGAAAAAT GAAGATGGAA 721 CATGGCCAAG GGGTCCTTCT ACTCCTAAAA AGCCACTAGA GCAGAGTTGC TACCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGATGCG CAGACGAGAC GCCTTTAGAA ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt